Y. Luo

AI-driven high-throughput droplet screening of cell-free gene expression

This paper introduces DropAI, an AI-driven droplet-based screening method to optimize cell-free gene expression systems, achieving cost reduction and yield improvement.

Global Marine Cold Seep Metagenomes Reveal Diversity of Taxonomy, Metabolic Function, and Natural Products

The paper analyzes metagenomes from cold seeps to create a database showing microbial diversity and potential natural products.

Directed Micro-Ecology Application System: On-site One-step Fermentation Facilitating Microbial Application through Theoretical and Equipment Innovation

The paper introduces a directed micro-ecology system with an intelligent fermentor that enhances microbial stability and effectiveness across multiple applications.

Mealtime alters daily rhythm in nuclear O-GlcNAc proteome to regulate hepatic gene expression

This study shows that mealtime affects the daily rhythm of nuclear O-GlcNAc proteome in mice, which regulates hepatic gene expression through interactions with phosphorylation.

MCP5, a methyl-accepting chemotaxis protein regulated by both the Hk1-Rrp1 and Rrp2-RpoN-RpoS pathways, is required for the immune evasion of Borrelia burgdorferi

This study identifies MCP5 as essential for Borrelia burgdorferi's immune evasion and transmission, regulated by two signaling pathways.

LncCE: Landscape of Cellular-Elevated LncRNAs in Single Cells Across Normal and Cancer Tissues

LncCE identifies and characterizes cellular-elevated lncRNAs across normal and cancer tissues using single-cell RNA sequencing data, providing expression patterns and clinical associations.

Comparative Analysis of the Techniques for the Determination of Binding Affinity between a Small Molecule Inhibitor and a Protein Target

This study compares KD values from SPR, ITC, and MST with Ki values from enzyme kinetics to assess their accuracy in determining binding affinity between small molecule inhibitors and protein targets.

Mapping Endothelial-Macrophage Interactions in Diabetic Vasculature: Role of TREM2 in Vascular Inflammation and Ischemic Response

This study maps endothelial-macrophage interactions in diabetic vasculature using single-cell and spatial transcriptomics, identifying TREM2 as a key driver of vascular inflammation and ischemic response in diabetes.

Genotypic-phenotypic landscape computation based on first principle and deep learning

The paper develops a deep learning framework to map genotypes to fitness, applies it to predict viral mutations and immune escape, and computes the genotype-fitness landscape for SARS-CoV-2.

Heparan sulfate selectively inhibits the collagenase activity of cathepsin K

This study shows heparan sulfate inhibits cathepsin K collagenase activity while preserving peptidase activity, revealing HS as a potential therapeutic target for bone resorption diseases.

Complementing Muscle Regeneration: Fibro-Adipogenic Progenitor and Macrophage-Mediated Repair of Elderly Human Skeletal Muscle

This study identifies fibro-adipogenic progenitors and macrophages as key players in elderly human skeletal muscle regeneration through spatial and single-cell transcriptomics, revealing their interplay via complement factor C3.

Generative Modelling of Oncogene-carrying Extrachromosomal Circular DNA Biogenesis and Dynamics in Cells

The paper develops a method to generate oncogene-carrying ecDNAs in human cells and studies their expression and stability under different conditions.

FLASH Irradiation Regulates IFN-β induction by mtDNA via Cytochrome c Leakage

This study shows FLASH irradiation increases cytochrome c leakage from mitochondria in normal cells, reducing mtDNA accumulation and IFN-β secretion, while having the opposite effect in cancer cells.

Caspase-8-mediated CYLD cleavage boots LPS-induced endotoxic shock.

This study identifies Caspase-8-mediated CYLD cleavage as a mechanism promoting LPS-induced endotoxic shock and reveals CP25 as a potential biomarker.

Advancing CAR T-Cell Therapy: Simultaneously Attack Tumor and Immunosuppressive Cells in the Tumor Microenvironment

This paper develops a PD-L1-targeting CAR T-cell therapy (MC9999) that effectively attacks both tumor and immunosuppressive cells in solid tumors.

Evolutionary trajectory of TRPM2 channel activation by adenosine diphosphate ribose and calcium

The paper traces the evolutionary changes in TRPM2 channel activation mechanisms across species, identifying key stages in the transition from simple to complex ligand binding and calcium gating.

Lipophilic compounds restore wt function of neurodevelopmental-associated KCNQ3 mutations.

The paper investigates how two KCNQ3 mutations impair channel function and shows that polyunsaturated fatty acids can restore wild-type function in these mutations.

Transcriptional Regulation of Protein Synthesis by Mediator Kinase in MYC-driven Medulloblastoma

This study identifies CDK8 as a critical dependence in MYC-driven medulloblastoma, showing its role in regulating transcription and translation through mechanisms involving ribosome biogenesis and 4EBP1 phosphorylation.

Resting natural killer cells promote the progress of colon cancer liver metastasis by elevating tumor-derived sSCF

The paper identifies resting NK cells that promote colon cancer liver metastasis by enhancing tumor-derived sSCF through cell-to-cell interactions.

The Bacillus subtilis yqgC-sodA operon protects magnesium-dependent enzymes by supporting manganese efflux

This paper shows that deletion of the yqgC-sodA operon in Bacillus subtilis causes Mn sensitivity by reducing Mn efflux and inhibiting Mg-dependent enzymes, distinct from efflux-deficient mutants.

The nanoscale organization of the Nipah virus fusion protein informs new membrane fusion mechanisms.

This study uses single-molecule microscopy to show NiV-F forms nanoclusters on membranes, which promote fusion by enabling interactions with the NiV-G/receptor complex.

Large-scale multi-omics analyses identified root-microbiome associations underlying plant nitrogen nutrition

The paper identifies root-microbiome associations affecting plant nitrogen nutrition through multi-omics analyses of 1,341 rapeseed samples.

HSP60 chaperone deficiency disrupts the mitochondrial matrix proteome and dysregulates cholesterol synthesis

This study investigates how HSP60 deficiency disrupts mitochondrial proteostasis and dysregulates cholesterol synthesis, leading to developmental abnormalities and hypomyelination.

Effects of different feeding methods on serum biochemical indexes, metabolic indexes, immune indexes, and intestinal microorganisms of Nanjiang yellow goats

This study examines how different feeding methods affect serum biochemical, immune, and metabolic indicators and intestinal microbiota in Nanjiang Yellow goats, finding significant differences in metabolites, immune factors, and microbial composition.

Metabolomics unveiled the impact of alterations in nucleotide glucose metabolism on palmitic acid-induced TLR4 activation in THP-1 macrophages

This study uses metabolomics to show that palmitic acid alters N-glycosylation-related metabolites in THP-1 macrophages, linking them to TLR4 activation and inflammation.

Brg1 regulates fibroblast-myofibroblast transition to promote renal fibrosis

This study shows Brg1 promotes fibroblast-myofibroblast transition in renal fibrosis and identifies Prune2 and PU.1 as downstream targets, with PFI-3 as a potential antifibrotic drug.

Plasmodesmata act as unconventional membrane contact sites regulating inter-cellular molecular exchange in plants

The paper shows plasmodesmata function as ER-PM tubular membrane contact sites that regulate intercellular molecular exchange in plants through PI4P and MCTP proteins.

New insights into the dynamics of de novo gene origin

This study identifies young, polymorphic de novo genes in Drosophila melanogaster by sequencing tissue-specific transcriptomes, revealing their transient nature and challenges in reconstructing their origin.

A Facile Method for Determining Lanthipeptide Stereochemistry

This study develops a simple method to determine lanthipeptide stereochemistry using Marfeys analysis with low sample requirements and standard equipment.

Cross-platform comparison of highly-sensitive immunoassays for inflammatory markers in a COVID-19 cohort

This study compares three immunoassay platforms for inflammatory markers in a COVID-19 cohort, finding differences in detectability, correlation, and differential expression.