M. Fang

Engineering probiotic Escherichia coli Nissle 1917 to block transfer of multiple antibiotic resistance genes by exploiting a type I CRISPR-Cas system

This paper engineers Escherichia coli Nissle 1917 to block antibiotic resistance gene transfer using a type I CRISPR-Cas system, showing efficacy in vitro and in zebrafish.

LrhA promotes CRISPR-Cas immunity by enhancing primed adaptation in Escherichia coli

This study identifies LrhA as a regulator that enhances CRISPR-Cas immunity in E. coli by promoting adaptation through increased spacer acquisition.

Inducible mismatch repair streamlines forward genetic approaches to target identification of cytotoxic small molecules

This study develops an inducible mismatch repair system to identify targets of orphan cytotoxins by screening for compound resistance mutations in engineered cell lines.

PI3Kδ inhibition potentiates glucocorticoids in B-ALL by decreased receptor phosphorylation and enhanced gene regulation

This study shows that inhibiting PI3Kδ enhances glucocorticoid efficacy in B-ALL by reducing GR phosphorylation and improving gene regulation.

Different tissues in the maternal-fetal interface harbor distinct microbiomes showing associations related to their anatomical position or function

The study analyzes microbiome profiles across maternal-fetal tissues, revealing distinct microbial compositions and correlations based on anatomical position.

Intranasal delivery of NS1-deleted influenza virus vectored COVID-19 vaccine restrains the SARS-CoV-2 inflammatory response

This study evaluates an intranasal influenza-based vaccine against SARS-CoV-2 variants, showing it reduces inflammatory response and tissue damage through local immunity.

Targeted Protein Degradation via Nanoparticles

This paper introduces a generic nanoparticle-based method for targeted protein degradation that avoids case-by-case design and enables degradation of both intracellular and extracellular targets.

Protective human anti-poxvirus monoclonal antibodies are generated from rare memory B cells isolated by multicolor antigen tetramers

The paper isolates rare memory B cells from vaccinated individuals to generate anti-poxvirus monoclonal antibodies, identifying one with high efficacy against viral infection.

RNA velocity unraveled

This paper analyzes RNA velocity methods, evaluates their assumptions, and proposes a framework for Markovian analysis to improve their accuracy and reliability.

Interpretable and tractable models of transcriptional noise for the rational design of single-molecule quantification experiments

Chemoproteomic profiling reveals cellular targets of nitro-fatty acids

This study identifies 184 nitro-alkylated proteins in human macrophages using a clickable nitro-fatty acid probe, revealing novel targets and pathways involved in nitro-fatty acid biology.

Transferrin receptor is another receptor for SARS-CoV-2 entry

This study identifies the transferrin receptor (TfR) as an additional receptor for SARS-CoV-2 entry, showing that TfR-mediated infection can occur independently of ACE2 and that targeting TfR has anti-viral effects.

Reconstitution of an intact clock that generates circadian DNA binding in vitro

The paper reconstitutes a cyanobacterial circadian clock in vitro to study its control over gene expression and provides a platform for synthetic biology.

AI-aided design of novel targeted covalent inhibitors against SARS-CoV-2

The paper designs novel covalent inhibitors against SARS-CoV-2 using a deep Q-learning network and fragment-based drug design, generating 47 lead compounds available in a public repository.

Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance

The paper shows that commensal microbes up-regulate host transferrin to suppress TLR signaling and promote immune tolerance in the gut.

Iron-deficiency and estrogen are associated with ischemic stroke by up-regulating transferrin to induce hypercoagulability

The paper shows that iron-deficiency and estrogen increase transferrin, leading to hypercoagulability and worsened ischemic stroke.

Metabolomics reveals that dietary xenoestrogens alter cellular metabolism induced by palbociclib/letrozole combination cancer therapy

The paper uses metabolomics to show that dietary xenoestrogens alter cellular metabolism in response to palbociclib/letrozole cancer therapy in MCF-7 cells.