K. Wu

Diffusing protein binders to intrinsically disordered proteins

The paper develops RFdiffusion to generate protein binders for IDPs/IDRs, achieving nanomolar affinity and functional applications in amylin and IL2RG.

Design of intrinsically disordered region binding proteins

The paper designs proteins that bind intrinsically disordered regions with high affinity and specificity across diverse targets.

Non-muscle myosin 2 can incorporate into established filaments in cells without an assembly competence domain

This study shows that non-muscle myosin 2A lacking the ACD can co-assemble with existing filaments, indicating the ACD is not required for association with established filaments.

RNA interactome of hypervirulent Klebsiella pneumoniae reveals a small RNA inhibitor of capsular mucoviscosity and virulence

This study identifies ArcZ as a small RNA inhibitor of capsular hypermucoviscosity and virulence in hypervirulent Klebsiella pneumoniae through RNA interactome profiling.

Biodegradation Potential of Neptunomonas naphthovoran - NAG-2N-126 for Crude Oil Pollution Mitigation: Experimental and Modelling Insights

This study evaluates the biodegradation potential of Neptunomonas naphthovoran NAG-2N-126 for crude oil pollution using experimental and mathematical modeling approaches, showing rapid growth and high aromatic compound degradation rates.

MAPK13 controls structural remodeling and disease after epithelial injury

This study identifies MAPK13 as a regulator of structural remodeling and disease after epithelial injury, showing its role in basal-epithelial stem cell hyperplasia and mucous metaplasia in both mouse and human models.

Discovery of orally bioavailable SARS-CoV-2 papain-like protease inhibitor as a potential treatment for COVID-19

The paper identifies a potent orally bioavailable SARS-CoV-2 PLpro inhibitor with in vitro and in vivo antiviral activity against SARS-CoV-2 and related coronaviruses.

BLOC1S1 control of vacuolar organelle fidelity modulates TH2 cell immunity and allergy susceptibility.

This study shows that BLOC1S1 depletion disrupts mitochondrial integrity, leading to TH2 cell immunity and allergy susceptibility via STING-NF-κB signaling.

Substrate recognition mechanism of the endoplasmic reticulum-associated ubiquitin ligase Doa10

This study defines the substrate recognition mechanism of Doa10 using structural and functional analyses in yeast, revealing a lateral tunnel and fence-like domains that facilitate substrate access.

Target-aware Molecule Generation for Drug Design Using a Chemical Language Model

This paper introduces TamGen, a chemical language model for target-aware molecule generation, and shows it produces high-quality compounds with inhibitory activity against Tuberculosis ClpP protease.

Solid tumor growth depends on an intricate equilibrium of malignant cell states

This study identifies a stereotypic organization of pancreatic ductal adenocarcinoma cells based on WNT signaling and Notch ligand interactions, showing that disrupting this equilibrium suppresses tumor growth and metastasis.

Structure basis for recognition of 26RFa by the pyroglutamylated RFamide peptide receptor

This study determines the cryo-EM structure of QRFPR bound to 26RFa, revealing the recognition mechanism and aiding drug design for obesity and diabetes.

The rapid diversification of Boletales is linked to Early Eocene and Mid-Miocene Climatic Optima

This study links the rapid diversification of Boletales fungi to two climatic optima, showing their expansion coincided with warm-humid climates and host plant diversification.

Resident memory T cell development is associated with AP-1 transcription factor upregulation across anatomical niches.

This study identifies AP-1 transcription factors as key regulators of TRM development across tissues using scRNA-seq and ATAC-seq, revealing a novel mechanism for TRM reactivation.

The HOMEODOMAIN-Like protein HDL mediates chromatin organization and rewires leaf epidermal patterning

This study identifies HDL, a homeodomain-like protein that regulates chromatin organization and influences leaf epidermal cell fate by modulating gene expression and cell heterogeneity.

De novo design of buttressed loops for sculpting protein functions

This paper designs proteins with structured loops supported by hydrogen bonds, demonstrating their stability and functionality in binding extended peptides.

Human receptive endometrial assembloid for deciphering the implantation window

This study develops a human endometrial assembloid model that mimics the receptive endometrium during the implantation window, showing structural and molecular features and hormone responsiveness.

Butyrate Protects against SARS-CoV-2-induced Tissue Damage in Golden Hamsters.

This study shows butyrate protects against SARS-CoV-2-induced tissue damage in golden hamsters by enhancing antiviral immunity and reducing oxidative stress.

A conserved and druggable pocket in class B G protein coupled receptors for orally active small molecule agonists

This study identifies a conserved binding pocket in class B GPCRs that enables small molecule agonists like PCO371 to activate G proteins without triggering arrestin signaling.

A potent MAPK13-14 inhibitor prevents airway inflammation and mucus production

This paper develops a potent MAPK13-14 inhibitor, NuP-3, which reduces airway inflammation and mucus production in human airway models and minipig disease models.

Cytosolic iron-sulfur protein assembly system identifies clients by a C-terminal tripeptide

The paper identifies a C-terminal tripeptide that directs Fe-S cluster insertion into cytosolic and nuclear proteins by binding to the CIA-targeting complex.

Homocysteine accelerates hepatocyte autophagy by upregulation of TFEB via DNMT3b-mediated DNA hypomethylation

This study shows homocysteine increases hepatocyte autophagy by upregulating TFEB through DNMT3b-mediated DNA hypomethylation of the TFEB promoter.

Arabidopsis TRB proteins function in H3K4me3 demethylation by recruiting JMJ14

This study shows TRB proteins recruit JMJ14 to demethylate H3K4me3 and coordinate with PRC2 to repress target genes.

Micromagnetic Stimulation (μMS) Dose-Response of the Rat Sciatic Nerve

This study investigates how varying magnetic field strength and frequency from a MagPen affects rat sciatic nerve activation, finding that higher frequencies require smaller amplitudes to trigger hind limb movement.

De novo design of modular peptide binding proteins by superhelical matching

This paper introduces a method to design modular peptide binding proteins that bind repeating peptide sequences with high specificity and affinity.

Structure of a G protein-coupled receptor with GRK2 and a biased ligand

The paper reports the structure of NTSR1 bound to GRK2, Gq, and a biased ligand, revealing how the ligand enhances GRK2 binding and enables arrestin-biased signaling.

Development of SARS-CoV-2 mRNA vaccines encoding spike N-terminal and receptor binding domains

The paper develops and evaluates an mRNA vaccine, mRNA-1283, encoding SARS-CoV-2 spike N-terminal and receptor-binding domains, showing improved immunogenicity and protection against multiple variants in mice.

An alternative mechanism for skeletal muscle dysfunction in long-term post-viral lung disease

This study identifies a distinct pattern of skeletal muscle dysfunction in a mouse model of long-term post-viral lung disease caused by Sendai virus, showing reduced myofiber size without changes in myofiber type or protein synthesis/degradation markers.

Intranasal delivery of NS1-deleted influenza virus vectored COVID-19 vaccine restrains the SARS-CoV-2 inflammatory response

This study evaluates an intranasal influenza-based vaccine against SARS-CoV-2 variants, showing it reduces inflammatory response and tissue damage through local immunity.

Genetic Analysis of Activin/Inhibin β Subunits in Zebrafish Development and Reproduction

This study investigates the role of activin/inhibin β subunits in zebrafish development and reproduction, showing their critical role in folliculogenesis and ovarian homeostasis.