L. Yi

A new yeast-based bioreporter for simple, sensitive, and cost-effective detection of dioxin-like compounds

This study develops a yeast-based bioreporter, DnaSc, for sensitive and cost-effective detection of dioxin-like compounds and AhR agonists with a detection limit of 10 fM for TCDD.

Dihydromyricetin promotes GLP-1 secretion to improve insulin resistance via "gut microbiota-CDCA"

This study shows that dihydromyricetin improves insulin resistance by enhancing GLP-1 secretion through the gut microbiota-CDCA pathway in HFD-induced mice.

Characterizing interactions of ER resident proteins in situ through the YST-PPI method

The paper introduces YST-PPI, a method to study ER resident protein interactions in situ, identifies 74 protein pairs, and highlights Lhs1p's role in regulating these interactions.

A Combinatorial Strategy for HRV 3C Protease Engineering to Achieve the N-terminal Free Cleavage

This paper develops a combinatorial strategy to engineer HRV 3C protease for N-terminal free cleavage by expanding P1 specificity through structure-guided library design and high-throughput screening.

Chalkophomycin Biosynthesis Revealing Unique Enzyme Architecture for a Hybrid Nonribosomal Peptide Synthetase and Polyketide Synthase

The paper identifies and characterizes the chm biosynthetic gene cluster responsible for chalkophomycin production in Streptomyces sp. CB00271 and proposes a biosynthesis model based on sequence analysis and structural modeling.

Successful spread of mcr-1-bearing IncX4 plasmids is associated with variant in replication protein of IncX4 plasmids

The paper shows that IncX4 plasmids with pir-1 have higher mcr-1 expression and fitness burden but lower invasion rates compared to pir-2 plasmids, explaining why mcr-1 is absent from pir-2 plasmids.

Computational design of N-linked glycans for high throughput epitope profiling

The paper develops a computational method using N-linked glycans to rapidly map epitopes, validated through ELISA, yeast display, and X-ray crystallography using human coagulation factor IXa.

Subchronic oral toxicity study of Aldicarb sulfoxide in Sprague-Dawley rats

This study examines the subchronic oral toxicity of Aldicarb sulfoxide in rats, finding liver injury and establishing safety thresholds for exposure.

Tumor suppressor p53 deficiency increases tumor immunogenicity through promoting IL33-mediated anti-tumor immune responses

This study shows that p53 deficiency in tumors enhances immunogenicity by increasing IL-33, which improves response to PD-1 ICB therapy in a mouse model.

Spatial compartmentalization of signalling imparts source-specific functions on secreted factors

The paper identifies distinct functions of VEGFA from different cell types in skeletal muscle regeneration through time-resolved transcriptomic analysis and functional experiments.

Proteomic Dissection of a Giant Cell

The paper quantifies polarized protein localization in Stentor coeruleus by cutting cells along the anterior-posterior axis and analyzing proteomic enrichment, finding 30% of proteins show polarized localization.

PixR, a novel activator of conjugative transfer of IncX4 resistance plasmids, mitigates the fitness cost of mcr-1 carriage in Escherichia coli

This study identifies PixR as a novel activator of IncX4 plasmid conjugation, showing it reduces the fitness cost of mcr-1 carriage in E. coli.

A novel approach to comparative RNA-Seq does not support a conserved set of genes underlying animal regeneration

This study uses a novel statistical method to analyze RNA-seq data from six animal regeneration datasets, finding that conserved gene clusters are enriched in structural genes rather than morphogenesis regulators.

The parathyroid hormone-dependent activation of osteoblasts enhances hematopoietic stem cell migration and reduces their engraftment abilities

This study shows that PTH-dependent osteoblast activation increases LT-HSC migration but reduces their engraftment ability in irradiated bone marrow.

After migration into blood circulation, hematopoietic stem cells can stably self-renew and maintain bone marrow while being skewed toward myeloid lineage when submitted to serial transplantation.

This study shows that migratory HSCs can self-renew and maintain BM but have reduced lymphoid reconstitution in serial transplants.

A valid protective immune response elicited in rhesus macaques by an inactivated vaccine is capable of defending against SARS-CoV-2 infection

This study shows an inactivated SARS-CoV-2 vaccine induces neutralizing antibodies and CTL responses in rhesus macaques, effectively preventing viral replication and lung inflammation.

Global Landscape of Native Protein Complexes in Synechocystis sp. PCC 6803

The paper identifies 291 protein complexes and 24,092 PPIs in Synechocystis sp. PCC 6803, revealing functional roles of previously uncharacterized proteins and their interactions across biological processes.

Sporadic ERK pulses drive non-genetic resistance in drug-adapted BRAFV600E melanoma cells

The paper shows that sporadic ERK pulses in BRAFV600E melanoma cells drive non-genetic resistance to RAF/MEK inhibitors.

Characterization and repurposing of the endogenous Type I-F CRISPR-Cas system of Zymomonas mobilis for genome engineering

This study characterizes and repurposes the endogenous Type I-F CRISPR-Cas system of Zymomonas mobilis to create an efficient genome engineering toolkit with 100% efficiency in various genetic modifications.

Molecular evolution, diversity and adaptation of H7N9 influenza A viruses in China

The paper analyzes the evolution and adaptation of H7N9 influenza viruses in China, identifying lineage-specific mutations and high-pathogenicity strains.