F. Gao

FLASH radiotherapy using high-energy X-rays: validation of the FLASH effect triggered by a compact single high-energy X-ray source device

This study validates the FLASH effect using a compact high-energy X-ray source in mice, showing protective effects on normal tissues but no significant tumor response differences between FLASH and conventional radiotherapy.

Strawberry COP9 signalosome FvCSN5A regulates plant development and fruit ripening by facilitating polyamine oxidase FvPAO5 degradation to control polyamine and H2O2 homeostasis

This study identifies FvCSN5A as a regulator of polyamine and H2O2 homeostasis in strawberries by controlling FvPAO5 degradation, affecting plant development and fruit ripening.

Synergically enhanced anti-tumor immunity of in vivo CAR by circRNA vaccine boosting

This study develops circRNA-based in vivo CAR and vaccines that synergistically enhance anti-tumor immunity in mice.

Myocardial infarction creates a critical time window for AAV gene therapy

This paper shows that myocardial infarction creates a time window where AAV gene therapy is more effective in mice, improving gene expression and therapeutic outcomes when administered three days post-infarction.

An orphan viral genome with controversial evolutionary status sheds light on a distinct lineage of flavi-like virus infecting plants

This study resolves the taxonomic controversy of SRAV, a plant-infecting flavi-like virus, by showing it belongs to the Flaviviridae family and triggers host RNAi responses.

Structural insights into transcription regulation of the global virulence factor PhoP from Mycobacterium tuberculosis

This study determines cryo-EM structures of PhoP-dependent transcription complexes in Mtb to elucidate its dual role as activator and repressor in stress adaptation.

The phylogenetic position of the Yunxian cranium elucidates the origin of Homo longi and the Denisovans

The study reconstructs the Yunxian 2 cranium and shows it belongs to the Homo longi clade, likely including Denisovans, and is closely related to the H. sapiens clade.

SHOOTMERISTEMLESS autoactivation: A prerequisite for fruit metamorphosis

The paper shows that CrSTM autoactivation through a cis regulatory element is essential for Capsella fruit shape transition during metamorphosis.

Camel milk affects serum metabolites by modulating the intestinal microflora

This study shows that camel milk modulates gut microbiota and serum metabolites in rats, with beneficial bacteria and metabolites resembling those of healthy individuals.

Patient-derived tumor organoids from resected non-small cell lung cancers for high-throughput response testing with approved and repurposed drugs

This study develops patient-derived tumor organoids from non-metastatic NSCLC tumors to test drug responses and identify chemoresistance mechanisms, finding 50% of organoids were chemo-resistant and could be sensitized by repurposed drugs.

Conservation of the ancestral function of GTSF1 in transposon silencing in unicellular Paramecium

This study shows that GTSF1 functions as a PIWI cofactor in transposon silencing in the unicellular eukaryote Paramecium, conserving an ancestral role observed in metazoans.

The role of neutrophil typing in homeostatic resilience protective model of inflammatory preconditioning

This study investigates the role of IRF4 and IRF5 in neutrophil response during septic peritonitis, showing their involvement in PMN phenotype switching and inflammatory outcomes.

Low- and high-grade glioma endothelial cells differentially regulate tumor growth

This study compares tumor endothelial cells from low-grade and high-grade gliomas, finding they have distinct molecular profiles and functional roles in regulating tumor growth.

MicroRNA-122-mediated liver detargeting enhances the tissue specificity of cardiac genome editing

This study shows that AAV-Tnnt2 vectors leak into the liver, reducing cardiac specificity, and demonstrates that adding miR122 target sequences reduces this leakiness.

Structural Basis of σ54 Displacement and Promoter Escape in Bacterial Transcription

This study presents cryo-EM structures of σ54 with increasing RNA length, revealing the mechanism of σ54 displacement and promoter escape during bacterial transcription initiation.

Alpaca Single B Cell Interrogation and Heavy-Chain-Only Antibody Discovery on an Optofluidic Platform

This paper develops a method to discover heavy-chain-only antibodies from alpaca B cells using an optofluidic platform and validates PSMA-specific VHH candidates.

Enhanced transmissibility of XBB.1.5 is contributed by both strong ACE2 binding and antibody evasion

This study shows XBB.1.5 has higher ACE2 binding and moderate antibody evasion, contributing to its increased transmissibility compared to other variants.

Runx factors launch T-cell and innate lymphoid programs via direct and gene network-based mechanisms

The paper shows Runx factors regulate T-cell and innate lymphoid programs through dynamic binding and gene network interactions during early development.

Compounds derived from N,N-dimethyldithiocarbamate are effective copper-dependent antimicrobials against Streptococcus pneumoniae

The paper develops and tests novel DMDC-derived compounds as copper-dependent antimicrobials against Streptococcus pneumoniae, showing effectiveness in vitro and in vivo.

Tumor suppressor p53 deficiency increases tumor immunogenicity through promoting IL33-mediated anti-tumor immune responses

This study shows that p53 deficiency in tumors enhances immunogenicity by increasing IL-33, which improves response to PD-1 ICB therapy in a mouse model.

Multi-Molecular Hyperspectral PRM-SRS Imaging

The paper introduces PRM-SRS imaging to visualize and identify multiple lipid species in cells and tissues with high chemical specificity and subcellular resolution.

PERK-ATAD3A interaction protects mitochondrial proteins synthesis during ER stress

The paper identifies ATAD3A as a PERK interactor that modulates PERK-eIF2 signaling during ER stress, allowing selective translation of mitochondrial proteins.

A novel quantification method for RPE phagocytosis using a VLC-PUFA-based strategy

This paper introduces a VLC-PUFA-based method to quantify RPE phagocytosis, focusing on lipid processing, validated against traditional methods.

Genetic Diversity of Candidatus Liberibacter asiaticus Based on Four Hypervariable Genomic Regions in China

This study analyzes genetic diversity of Candidatus Liberibacter asiaticus in China using hypervariable genomic regions to understand strain differences and inform HLB control strategies.

SARS-CoV-2 variant B.1.1.7 caused HLA-A2+ CD8+ T cell epitope mutations for impaired cellular immune response

This study identifies mutations in SARS-CoV-2 variant B.1.1.7 that reduce HLA-A2 restricted CD8+ T cell epitope binding and impair cellular immune responses.

Introducing an argonaute-facilitated PCR platform

This paper introduces an argonaute-facilitated PCR platform that improves PCR sensitivity by using MejAgo to enhance guide DNA/template pairing, eliminating the need for an annealing step.

Vertical HIV-1 transmission in the setting of maternal broad and potent antibody responses

This study examines maternal broadly neutralizing antibody (bnAb) specificities in HIV-transmitting and non-transmitting mothers to determine if certain bnAb specificities drive infant transmitted/founder virus selection.

Impact of various vaccine boosters on neutralization against Omicron following prime vaccinations with inactivated or adenovirus-vectored vaccine

This study evaluates the effectiveness of various vaccine boosters against the Omicron variant following prime vaccinations with inactivated or adenovirus-vectored vaccines, finding that certain boosters significantly enhance neutralizing activity.

cpSRP43 is both highly flexible and stable: Structural insights using a combined experimental and computational approach

This study investigates the structural stability and flexibility of cpSRP43 using biophysical techniques and molecular dynamics simulations, revealing it is both highly stable and flexible.

G2 stem cells orchestrate time-directed, long-range coordination of calcium signaling during skin epidermal regeneration

The paper uses a new machine learning tool to analyze calcium signaling dynamics in epidermal stem cells during regeneration, revealing that G2 stem cells coordinate long-range signaling through gap junctions.