N. Wu

rarestR: An R package using rarefaction metrics to estimate α-diversity (species richness) and β-diversity (species shared) for incomplete samples

rarestR estimates α- and β-diversity for incomplete samples using rarefaction metrics and parametric extrapolations, offering visualization tools for curve fitting.

Differences in milk microbiota between healthy cows and cows with recurring Klebsiella mastitis

The paper compares milk microbiota between healthy cows and those with recurring Klebsiella mastitis, finding differences in bacterial genera associated with infection.

Metabolomics unveiled the impact of alterations in nucleotide glucose metabolism on palmitic acid-induced TLR4 activation in THP-1 macrophages

This study uses metabolomics to show that palmitic acid alters N-glycosylation-related metabolites in THP-1 macrophages, linking them to TLR4 activation and inflammation.

Collagen co-localised with macrovesicular steatosis for fibrosis progression in non-alcoholic fatty liver disease

This study quantifies steatosis and fibrosis progression in NAFLD models, finding that collagen co-localized with macrosteatosis better differentiates fibrosis stages.

Single-cell Transcriptomic Analysis of Systemic Autoinflammatory Diseases with Anti-TNFα Therapy

This study uses single-cell RNA sequencing to analyze PBMCs and PMNs in SAIDs patients before and after anti-TNF therapy, revealing interferon responses in disease activity and unexpected cytokine elevation post-treatment.

Mode of inhibition of RNase P by gambogic acid and juglone

This study identifies gambogic acid and juglone as inhibitors of PRORP1, with distinct mechanisms of action, and shows they also inhibit human mtRNase P.

Biophysical analysis of Gaussia Luciferase bioluminescence mechanisms using a non-oxidizable coelenterazine.

The paper investigates the bioluminescence mechanism of Gaussia Luciferase using azacoelenterazine, revealing the role of salt and monovalent anions and structural changes in catalysis.

Omicron-specific mRNA vaccine elicits potent immune responses in mice, hamsters, and nonhuman primates

This study develops an Omicron-specific mRNA vaccine that shows superior neutralizing antibody responses in mice, hamsters, and macaques compared to existing vaccines.

In situ architecture and membrane fusion of SARS-CoV-2 Delta variant

The paper reports the in situ structure of SARS-CoV-2 Delta variant virions and captures membrane fusion snapshots, revealing unique architectural features and fusion mechanisms.

SER: an R package to compute environmental regime over a certain time period

SER computes environmental regimes over time periods to improve understanding of environmental impacts on ecological communities and other fields.

Combined SHP2 and ERK inhibition for the treatment of KRAS-driven Pancreatic Ductal Adenocarcinoma

This paper investigates the combination of SHP2 and ERK inhibition as a treatment for KRAS-driven pancreatic ductal adenocarcinoma, showing synergistic anticancer effects and tumor regression in mouse models.

Molecular Epidemiology and Microbiological Characteristics of Cryptococcus gattii VGII isolates from China

This study analyzes C. gattii VGII isolates from China using MLST and WGS, revealing genetic diversity and virulence differences, with no drug resistance observed.

The gut microbial signature of gestational diabetes mellitus and the association with diet intervention

This study examines gut microbiota composition in GDM patients before and after diet intervention, finding that diet affects the Firmicutes/Bacteroidetes ratio and specific taxa rather than altering the overall microbial pattern.

Loss of MGA mediated Polycomb repression promotes tumor progression and invasiveness

This study shows that loss of MGA promotes tumor growth and invasiveness by de-repressing Polycomb targets in lung and colon cancers.

ROR and RYK extracellular region structures suggest that receptor tyrosine kinases have distinct WNT-recognition modes

The paper presents crystal structures of Drosophila ROR and RYK orthologs, revealing distinct WNT-recognition mechanisms through their extracellular domains.

Understanding the phase separation characteristics of nucleocapsid protein provides a new therapeutic opportunity against SARS-CoV-2

This study identifies that SARS-CoV-2 nucleocapsid protein undergoes phase separation, which can be modulated by small molecules, offering new therapeutic strategies against COVID-19.

Characterization of a glycolipid glycosyltransferase with broad substrate specificity from the marine bacterium Candidatus Pelagibacter sp. HTCC7211

This paper characterizes a glycolipid glycosyltransferase (GTcp) from Candidatus Pelagibacter sp. HTCC7211, showing its broad substrate specificity and role in glycoglycerolipid synthesis under phosphorus stress.

Integrative Transcriptomic Analysis Reveals the Immune Mechanism for A CyHV-3-Resistant Common Carp Strain

This study identifies immune mechanisms underlying CyHV-3 resistance in a common carp strain through transcriptomic analysis of gene and lncRNA expression.

Molecular architecture of the SARS-CoV-2 virus

This study reveals the molecular architecture of the SARS-CoV-2 virus using cryo-ET and STA, showing the structure of S proteins, N-linked glycans, and RNPs.

Solution structure of Gaussia Luciferase with five disulfide bonds and identification of a putative coelenterazine binding cavity by heteronuclear NMR

This paper determines the solution structure of Gaussia Luciferase using NMR and identifies a potential coelenterazine binding cavity involved in bioluminescence.

Map3k2-Regulated Intestinal Stromal Cells (MRISC) Define a Distinct Sub-cryptic Stem Cell Niche for Damage Induced Wnt Agonist R-spondin1 Production

The paper identifies MRISC cells as a novel intestinal stem cell niche that produces R-spondin1 via Map3k2 signaling to promote intestinal regeneration after damage.

Kinetic mechanism of human mitochondrial RNase P

This study investigates the kinetic mechanism of human mitochondrial RNase P, showing that MRPP1/2 enhances catalytic activity and substrate affinity, and facilitates MRPP3 recognition and cleavage efficiency.

Selection of a Malignant Subpopulation from a Colorectal Cancer Cell Line

This study develops a sphere-forming assay to isolate a malignant subpopulation from a CRC cell line and identifies DPEP1 as a prognostic biomarker.