L. Fang

Measurement precision bounds on aberrated single molecule emission patterns

This study quantifies the precision limits of position and wavefront distortion measurements in SMLM under various aberrations, providing theoretical guidelines for aberration correction.

RNA Infrastructure Profiling Illuminates Transcriptome Structure in Crowded Spaces

The paper introduces RNA infrastructure profiling (RISP) to analyze RNA structure and modifications in crowded cellular environments, showing improved coverage and m6A detection with small acetyl probes.

β-catenin inhibition disrupts the homeostasis of osteogenic/adipogenic differentiation leading to the development of glucocorticoid-induced osteonecrosis of femoral head

This study shows that β-catenin inhibition disrupts osteogenic/adipogenic differentiation in bone marrow mesenchymal cells, contributing to glucocorticoid-induced osteonecrosis of the femoral head.

Geranylgeranyl pyrophosphate (GGPP) is associated with hepatic lipid accumulation and insulin resistance in MAO by prenylating Perilipin4

This study shows GGPP promotes hepatic lipid accumulation and insulin resistance in MAO by prenylating Perilipin4, and inhibiting GGPP synthase reduces these effects.

Spatial transcriptomic atlas of shoot organogenesis in tomato callus

This study creates a spatial transcriptomic atlas of tomato callus during shoot regeneration, identifying cell types and developmental trajectories.

Born with intronless ERF transcriptional factors: C4 photosynthesis inherits a legacy dating back 450 million years

This study identifies intronless ERF transcription factors as a molecular tool used in the evolution of C4 photosynthesis in multiple plant lineages.

Depletion of collagen IX alpha2 interferes osteochondral homeostasis of the knee joint which ultimately causing osteoarthritis-like articular cartilage damage

This study shows that Col92 deficiency in mice leads to osteochondral homeostasis disruption and osteoarthritis-like cartilage damage in the knee joint.

Pervasive Transcriptome Interactions of Protein-Targeted Drugs

This study develops a method to assess RNA off-target interactions of protein-targeted drugs and finds they pervasively affect the transcriptome, potentially causing toxicity.

Tumor-derived biomarkers beyond antigen expression enhance efficacy of CD276/B7H3 antibody-drug conjugate in metastatic prostate cancer

This study identifies biomarkers beyond B7H3 expression that predict response to a B7H3-ADC in metastatic prostate cancer models, improving therapeutic efficacy.

Real-time denoising of fluorescence time-lapse imaging enables high-sensitivity observations of biological dynamics beyond the shot-noise limit

This paper introduces DeepCAD-RT, a real-time denoising method for fluorescence time-lapse imaging that improves signal-to-noise ratio with fewer photons, demonstrated across multiple organisms and biological processes.

Rapid deep widefield neuron finder driven by virtual calcium imaging data

The paper introduces DeepWonder, a deep learning tool for rapid and accurate neuron detection in widefield calcium imaging data, achieving faster processing and improved signal-to-background ratio compared to traditional methods.

Neural stemness unifies cell tumorigenicity and pluripotent differentiation potential

The paper shows that neural stemness unifies tumorigenicity and pluripotent differentiation potential by demonstrating SETDB1's role in maintaining these properties through epigenetic and transcriptional mechanisms.

Profiling of the drug resistance of thousands of Src tyrosine kinase mutants uncovers a regulatory network that couples autoinhibition to catalytic domain dynamics

DEAD-box RNA helicase 18 disrupts IRF3-binding to the interferon-β promoter

This study identifies DDX18 as a negative regulator of IRF3 binding to the IFN-β promoter, suppressing type I IFN production during viral infection.

Drought exerts a greater influence than growth temperature on the temperature response of leaf day respiration in wheat (Triticum aestivum)

This study shows drought has a greater impact than growth temperature on wheat leaf respiration temperature response and highlights the inaccuracy of the Kok method for estimating respiration.

Investigating the active substance and mechanism of Jing-Fu-Kang granules via mass spectrometry technology and network pharmacology method

This study identifies 97 chemical constituents of Jing-Fu-Kang granules and reveals 531 common targets and 136 pathways involved in its mechanism for cervical spondylosis using mass spectrometry and network pharmacology.

Reduced neutralization of SARS-CoV-2 B.1.617 variant by inactivated and RBD-subunit vaccine

The paper evaluates the neutralization efficacy of inactivated and RBD-subunit vaccines against SARS-CoV-2 B.1.617 and B.1.1.7 variants using a pseudovirus-based assay.

3D observation of large-scale subcellular dynamics in vivo at the millisecond scale

This paper introduces a method to observe 3D subcellular dynamics in vivo at high resolution and speed, demonstrating its application in various biological systems.

Spike Protein Targeting "Nano-Glue" that Captures and Promotes SARS-CoV-2 Elimination

This paper discovers CIPS nanosheets that selectively bind SARS-CoV-2 spike protein, inhibit viral entry, and promote macrophage-mediated viral elimination.

Emerging SARS-CoV-2 variants reduce neutralization sensitivity to convalescent sera and monoclonal antibodies

This study evaluates the neutralization sensitivity of SARS-CoV-2 variants N501Y.V1 and N501Y.V2 to convalescent sera and monoclonal antibodies, finding reduced neutralization activity.

Coordinated regulation of ribosomes and proteasomes by PRMT1 in the maintenance of neural stemness of cancer cells and neural stem cells

The paper shows PRMT1 maintains neural stemness by coordinating ribosomes and proteasomes through arginine methylation, preventing degradation and supporting protein homeostasis in cancer and neural stem cells.

Single-cell multi-omics reveals elevated plasticity and stem-cell-like blasts relevant to the poor prognosis of KMT2A-rearranged leukemia

This study uses single-cell multi-omics to identify lineage plasticity and stem-cell-like blasts in KMT2A-rearranged leukemia, linking these features to poor prognosis in infants.

In vitro culture of aberrant basal-like cells from fibrotic lung tissue

The paper cultures and characterizes aberrant basal-like cells from fibrotic lung tissue, finding they co-express epithelial and mesenchymal markers and show disease-specific transcriptomic differences between IPF and other ILD patients.

Combination of ipratropium bromide and salbutamol in children and adolescents with asthma: a meta-analysis

This study evaluates the efficacy and safety of combining ipratropium bromide and salbutamol in children and adolescents with asthma, finding reduced hospital admissions but no significant adverse event differences.

FGFR Signaling Coordinates the Balance Between Glycolysis and Fatty Acid β-Oxidation in Lymphatic Endothelial Cells

This study shows FGFR signaling regulates the balance between glycolysis and fatty acid β-oxidation in lymphatic endothelial cells, with FGFR inhibition increasing FAO and impairing cell proliferation.

Immunoglobulin fragment F(ab')2 against RBD potently neutralizes SARS-CoV-2 in vitro

This study produces and tests an F(ab')2 fragment against SARS-CoV-2 RBD, showing potent in vitro neutralization with an EC50 of 0.07 g/ml.

Role of RgsA in oxidative stress resistance in Pseudomonas aeruginosa

This study shows that deletion of RgsA in Pseudomonas aeruginosa reduces growth and oxidative stress resistance in both planktonic and biofilm states.

Genome-wide targets identification by CRISPRi-Omics for high-titer production of free fatty acids in Escherichia coli

The paper identifies 56 beneficial genes for optimizing free fatty acids production in E. coli using CRISPRi-Omics and reports a record FFAs titer of 21.6 g L-1.

Augmenting Vascular Disease Diagnosis by Vasculature-awareUnsupervised Learning

This paper introduces VasNet, an unsupervised learning algorithm that improves vascular disease diagnosis from unlabeled images by focusing on retinal vasculature features.

Tuft cells restrain pancreatic tumorigenesis through paracrine eicosanoid signaling

This study shows that tuft cells suppress pancreatic tumorigenesis by producing anti-inflammatory prostaglandin D2, which inhibits stromal activation, and identifies their protective role in early disease progression.