T. Hu

Parallel Analyses by Mass Spectrometry (MS) and Reverse Phase Protein Array (RPPA) Reveal Complementary Proteomic Profiles in Triple-Negative Breast Cancer (TNBC) Patient Tissues and Cell Cultures

The paper compares Mass Spectrometry (MS) and Reverse Phase Protein Array (RPPA) in TNBC samples to assess their performance and identify complementary proteomic profiles.

Multiple Unfolded Protein Response pathways cooperate to link cytosolic dsDNA release to Stimulator of Interferon Gene (STING) activation

This study identifies multiple UPR pathways that cooperate to link cytosolic dsDNA release to STING activation and IFN-β induction in response to ER stress.

Automated muscle path calibration with gradient-specified optimization based on moment arm

This paper introduces a gradient-specified optimization method to calibrate muscle paths based on desired moment arms, achieving fast and accurate replication of muscle paths from a shoulder-arm model.

ZNF574 is a Quality Control Factor For Defective Ribosome Biogenesis Intermediates

The paper identifies ZNF574 as a key factor in a quality control pathway that degrades defective ribosome intermediates in human cells.

Multi-Niche Human Bone Marrow On-A-Chip for Studying the Interactions of Adoptive CAR-T Cell Therapies with Multiple Myeloma

This study develops a 3D bone marrow-on-a-chip model to investigate interactions between CAR-T cells and multiple myeloma cells in different niches and assess therapeutic responses.

Comparative single-cell regulome reveals evolutionary innovations in neural progenitor cells during primate corticogenesis

The paper compares single-cell regulomes of macaque and mouse neural progenitors with human data to identify evolutionary changes in cortex development.

MiR-574-5p activates human TLR8 to promote autoimmune signaling and lupus

This study shows miR-574-5p activates TLR8 to drive autoimmune signaling and lupus progression by enhancing cytokine production, STAT1 activation, and autoantigen generation.

Detection and characterization of a novel bat filovirus (Dehong virus, DEHV) in fruit bats

The paper detects and characterizes a novel bat filovirus, Dehong virus (DEHV), from Rousettus leschenaultii bats, placing it phylogenetically between Dianlovirus and Marburgvirus.

CD4+ T cell senescence is associated with reduced reactogenicity in severe/critical COVID-19

The paper finds that higher levels of CD4+ T cell senescence in severe/critical COVID-19 patients correlate with reduced antibody responses and worse survival, especially in breakthrough infections.

Pathogen-driven CRISPR screens identify TREX1 as a regulator of DNA self-sensing during influenza virus infection

The paper uses a CRISPR screen to identify TREX1 as a pro-viral host factor that modulates DNA sensing during influenza infection.

30-Minute Highly Multiplexed VaxArray Immunoassay for Pneumococcal Vaccine Antigen Characterization

The paper introduces a 30-minute multiplexed immunoassay for identifying and quantifying pneumococcal vaccine antigens with high specificity and precision.

Rapid Identity and Quantity CQA Test for Multivalent mRNA Drug Product Formulations

This paper demonstrates a multiplexed platform for rapid identity verification and concentration determination of mRNA formulations, achieving high specificity and sensitivity without PCR or mRNA extraction.

VaxArray Immunoassay for the Multiplexed Quantification of Poliovirus D-Antigen

The paper develops a multiplexed immunoassay for rapid and accurate quantification of poliovirus D-antigen across all three serotypes, improving upon traditional ELISA methods.

A bacterial genome and culture collection of gut microbial in weanling piglet

The paper establishes a bacterial genome and culture collection of the piglet gut microbiome, revealing new species and functional insights.

The Detection of Bergeyella zoohelcum in Young Children after Cat Bite

This paper reports the isolation and identification of Bergeyella zoohelcum from a cat-bite infected child, highlighting challenges in its detection and the importance of advanced techniques.

Identification of novel bat coronaviruses sheds light on the evolutionary origins of SARS-CoV-2 and related viruses

The paper identifies novel bat coronaviruses, including relatives of SARS-CoV-2, from a region in China, providing insights into their evolutionary origins and geographic distribution.

A Facile and Rapid Route to Self-Digitization of Samples into a High Density Microwell Array for Digital Bioassays

This paper introduces a rapid method for sample partitioning into microwells for digital bioassays, achieving high-density arrays with over 100,000 wells in under 30 seconds.

The Prognostic Value of Tumor-Infiltrating Immune Cells in Gynecologic Cancers

This study characterizes immune infiltration patterns in ovarian, cervical, and uterine cancers and identifies immune subtypes associated with prognosis.

Structural basis for repurpose and design of nucleoside drugs for treating COVID-19

The paper reports cryo-EM structures of SARS-CoV-2 RdRp with nucleoside inhibitors, revealing their mechanism and guiding the design of more potent derivatives.

Winter wheat adapts to environmental pH by changing H+ net flux in roots at the seedling stage

This study shows winter wheat adjusts root H+ net flux to adapt to pH stress, with BN4199 showing stronger acid and alkali resistance than AK58.

Implantable photonic neural probes for light-sheet fluorescence brain imaging

The paper introduces implantable photonic neural probes that enable light-sheet fluorescence imaging in brain tissues without additional micro-optic components.

Chloroquine and hydroxychloroquine as ACE2 blockers to inhibit viropexis of 2019-nCoV Spike pseudotyped virus

This study investigates whether chloroquine and hydroxychloroquine inhibit 2019-nCoV infection by blocking ACE2 binding, showing both drugs suppress virus entry into ACE2-expressing cells.

IP-MS Analysis of ESX-5 and ESX-1 Substrates Enables Mycobacterial Species Identification

The paper develops an IP-MS assay to identify slow-growing mycobacterial species in early MGIT cultures using species-specific antibodies.

Structural basis for the inhibition of SARS-CoV-2 main protease by antineoplastic drug Carmofur

This study shows Carmofur inhibits SARS-CoV-2 main protease by covalently binding to Cys145 and occupying the S2 subsite, with an EC50 of 24.30 M in cell-based viral replication assays.

Structure of RNA-dependent RNA polymerase from 2019-nCoV, a major antiviral drug target

The paper reports the cryo-EM structure of 2019-nCoV RdRp complexed with cofactors, revealing key residues and remdesivir binding, aiding antiviral drug design.

A novel bat coronavirus reveals natural insertions at the S1/S2 cleavage site of the Spike protein and a possible recombinant origin of HCoV-19

This paper identifies a novel bat coronavirus, RmYN02, with sequence similarities to HCoV-19 and natural insertions at the S1/S2 cleavage site, suggesting a possible recombinant origin for HCoV-19.

Brucella suppress innate immunity by down-regulating STING expression in macrophages

This study shows Brucella suppresses STING expression via miR-24 to evade innate immunity and establish chronic infection.

In-Depth Characterization and Validation in BRG1-Mutant Lung Cancers Define Novel Catalytic Inhibitors of SWI/SNF Chromatin Remodeling

This study validates novel BRM/BRG1 ATPase inhibitors as chemical probes for SWI/SNF chromatin remodeling and shows their therapeutic potential in BRG1-mut lung cancers.

IL-25 induces beige fat to improve metabolic homeostasis via macrophage and innervation

This study shows IL-25 promotes beige fat browning through macrophage activation and innervation, improving metabolic homeostasis in obese mice.

Hemolysin liberates bacterial outer membrane vesicles for cytosolic lipopolysaccharide sensing

This study shows hemolysin facilitates cytosolic LPS sensing by liberating bacterial outer membrane vesicles, leading to inflammasome activation and gut inflammation.