L. Wan

Single-Cell multiomics reveals ENL mutation perturbs kidney developmental trajectory by rewiring gene regulatory landscape

This study shows that an ENL mutation disrupts kidney development by altering gene regulation, leading to developmental defects and early death in mice, and suggests potential therapeutic strategies.

Force-Enhanced Sensitive Detection of New DNA-Interactive Agents from Microorganisms at the Single-Molecule Level

This paper introduces a single-molecule stretching assay to detect DNA-intercalating agents from microorganisms, identifying two strains and three compounds with anti-cancer activity.

The deubiquitinase USP9X regulates RIT1 protein abundance and oncogenic phenotypes

This study shows that USP9X stabilizes RIT1 protein and that its depletion reduces RIT1 abundance and sensitizes cells to EGFR inhibitors.

RFC1 AAGGG pentanucleotide repeats form parallel G-quadruplex: structural implications for aberrant molecular cascades in CANVAS

The paper identifies and structures the G-quadruplex formed by RFC1 AAGGG repeats, showing its role in replication stalling and transcription inhibition in CANVAS.

Preclinical Screening of Splice-Switching Antisense Oligonucleotides in PDAC Organoids

This study develops an efficient ASO transfection method to screen splicing events in PDAC organoids for therapeutic targets.

Plasma membrane association and resistosome formation of plant helper immune receptors

This study identifies the role of positively charged residues in helper NLRs for phospholipid binding and plasma membrane association, and shows effector activation induces NRG1 oligomerization at the plasma membrane.

SARS-CoV-2 spike-induced syncytia are senescent and contribute to exacerbated heart failure

This study links SARS-CoV-2 spike-induced syncytia to cellular senescence and exacerbated heart failure, showing that senolytic drugs and WNK1 inhibitors can mitigate cardiac damage.

Plant and prokaryotic TIR domains generate distinct cyclic ADPR NADase products

This study investigates whether prokaryotic Thoeris TIRs can cross-activate plant TIR-immunity and finds they generate overlapping cADPR isomers but have distinct signaling requirements.

p53 restoration in small cell lung cancer identifies a latent Cyclophilin-dependent necrosis mechanism

This study shows that p53 restoration in SCLC can induce necrosis via cyclophilin-dependent mechanisms, revealing subtype-specific responses and potential therapeutic targets.

Allelic variation in the Arabidopsis TNL CHS3/CSA1 immune receptor pair reveals two functional regulatory modes

The paper identifies two regulatory modes in the Arabidopsis CHS3/CSA1 immune receptor pair, showing how an integrated domain (ID) in clade 1 CHS3 enables effector binding, while other clades rely on detecting effector-modified pattern recognition receptors.

A system for multiplexed selection of aptamers with exquisite specificity without counter-selection

This paper introduces a high-throughput screening platform for generating highly specific aptamers without counter-selection, demonstrating its effectiveness with kynurenine metabolites.

Loss of KRASG12D feedback regulation involving splicing factor SRSF1 accelerates pancreatic cancer

The paper shows that loss of SRSF1, a splicing factor, accelerates pancreatic cancer by disrupting homeostasis and enhancing KrasG12D-driven tumorigenesis.

Mechanism of LolCDE as a molecular extruder of bacterial triacylated lipoproteins

This study determines the cryo-EM structures of LolCDE and reveals its mechanism for extracting triacylated lipoproteins from the cytoplasmic membrane.

Discovery of indole-modified aptamers for highly specific recognition of protein glycoforms

This paper develops indole-modified aptamers using FACS to specifically recognize protein glycoforms, achieving discrimination between structurally similar molecules differing only in glycan modifications.

The plant immune receptors NRG1.1 and ADR1 are calcium influx channels

The paper identifies NRG1.1 and ADR1 as plant immune receptors that function as calcium-permeable channels to induce cell death.

BiTSC^2: Bayesian inference of Tumor clonal Tree by joint analysis of Single-Cell SNV and CNA data

BiTSC2 jointly analyzes single-cell SNV and CNA data to infer tumor clonal trees with high accuracy and robustness to low sequencing depth.

Coiled-coil and RPW8-type immune receptors function at the plasma membrane in a phospholipid dependent manner

This study shows that NLR immune receptors require phospholipid interaction for plasma membrane localization and cell death activity.

SARS-CoV-2 manipulates the SR-B1-mediated HDL uptake pathway for its entry

This study shows SARS-CoV-2 uses the SR-B1-mediated HDL uptake pathway alongside ACE2 for entry, and blocking this pathway inhibits infection.

A novel biparatopic antibody-ACE2 fusion that blocks SARS-CoV-2 infection: implications for therapy

The paper develops a biparatopic antibody-ACE2 fusion (89C8-ACE2) that effectively blocks SARS-CoV-2 infection in vitro and neutralizes authentic virus at low nanomolar concentrations.

Flow-cell based technology for massively parallelcharacterization of base-modified DNA aptamers

This paper introduces a flow-cell based system for high-throughput characterization of base-modified DNA aptamers using a modified Illumina MiSeq instrument.

Molecular characterization of differences between the tomato immune receptors Fls3 and Fls2

This study compares the immune responses of tomato receptors Fls2 and Fls3, finding they contribute equally to disease resistance but differ in ROS production, gene expression, and kinase activity.

A revised 1.6 Å structure of the GTPase domain of the Parkinson’s disease-associated protein LRRK2 provides insights into mechanisms

This paper reports a 1.6 Å crystal structure of the GTPase domain of LRRK2, revealing how PD-associated mutations at Arg-1441 impair nucleotide-dependent conformational dynamics.

Bacillus thuringiensis targets the host intestinal epithelial junctions for successful infection of Caenorhabditis elegans

This paper shows Bacillus thuringiensis uses Cry toxins and PlcR-regulated virulence factors to disrupt C. elegans intestinal epithelial junctions, enabling successful infection.

Research Synergy and Drug Development: Bright Stars in Neighboring Constellations

The paper uses data mining and network analysis to study the collaborative networks behind five anti-cancer therapeutics, identifying key publications, awards, and peer review panels involved in their development.