N. Yan

Identification of Small Molecule Inhibitors of PPM1D Using a Novel Drug Discovery Platform

This study identifies new PPM1D inhibitors using a drug discovery platform and shows their distinct biological effects compared to other DDR and p53 pathway modulators.

Cryo-EM Structure of HRSL Domain Reveals Activating Crossed Helices at the Core of GCN2

The paper reports a cryo-EM structure of the HRSL domain from GCN2, revealing its role in activating the kinase and regulating the ISR pathway.

STING Licensing of Type I Dendritic Cells Potentiates Antitumor Immunity

A 'shock-and-lock' nanoparticle agonist activates STING in type 1 conventional dendritic cells to enhance antitumor immunity with associated biomarkers.

Addressing preferred orientation in single-particle cryo-EM through AI-generated auxiliary particles.

This paper introduces cryoPROS, an AI method to address preferred orientation in cryo-EM, improving resolution without special experimental techniques.

Structural basis for sterol sensing by Scap and Insig

This study determines the structure of the Scap/Insig-2 complex in the absence of 25HC, revealing structural insights into cholesterol sensing and regulatory mechanisms.

Prediction of occult tumor progression via platelet RNAs in a mouse melanoma model: a potential new platform of cancer screening for early detection of cancer

This paper identifies platelet RNAs that predict occult tumor progression in a mouse melanoma model, showing high diagnostic accuracy for early cancer detection.

Structure of human Nav1.5 reveals the fast inactivation-related segments as a mutational hotspot for the Long QT Syndrome

The paper presents the cryo-EM structure of the human Nav1.5-E1784K variant and identifies a mutational hotspot in fast inactivation segments linked to LQT3.

Long-term survival of salmon-attached SARS-CoV-2 at 4 degree as a potential source of transmission in seafood markets

This study shows that SARS-CoV-2 attached to salmon can remain infectious for over a week at 4°C, indicating a potential transmission risk in seafood markets.

Orthosteric-allosteric dual inhibitors of PfHT1 as selective anti-malarial agents

This paper develops dual orthosteric-allosteric inhibitors of PfHT1 that selectively target malaria parasites over human cells, showing potent activity against multidrug-resistant strains.

Employing NaChBac for cryo-EM analysis of toxin action on voltage-gated Na+ channels in nanodisc

The paper presents cryo-EM structures of NaChBac in nanodiscs and its complex with HuwenToxin IV, revealing toxin docking details and enabling studies of GMTs on Nav channels.

Structure and mechanism of a primate ferroportin

This study presents the structure of primate ferroportin and elucidates its mechanism of iron transport and regulation by hepcidin.

Structural basis for catalysis and substrate specificity of human ACAT1

The paper presents the cryo-EM structure of human ACAT1 and reveals its catalytic mechanism and substrate preference for unsaturated acyl chains.

Structure and mechanism of human diacylglycerol acyltransferase 1

This study determines the structure of hDGAT1 with a substrate, revealing its homodimeric organization and substrate-binding sites.

Structural basis for pore blockade of the human cardiac sodium channel Nav1.5 by tetrodotoxin and quinidine

The paper presents cryo-EM structures of Nav1.5 bound to tetrodotoxin and quinidine, revealing mechanisms of pore blockade and disease mutation mapping.

High Yield Monolayer Graphene Grids for Near-Atomic Resolution Cryo-Electron Microscopy

This paper introduces a method for preparing high-yield monolayer graphene cryo-EM grids that improve resolution and protein density for cryo-EM studies.

Metabolic Control of Viral Infection through PPAR-α Regulation of STING Signaling

This study shows that PPAR-α activation increases herpesvirus replication, suppresses type I interferon, and inhibits STING signaling through ROS.

Molecular basis for allosteric regulation of the type 2 ryanodine receptor channel gating by key modulators

The paper reports cryo-EM structures of RyR2 bound to modulators, revealing how Ca2+ and other molecules regulate channel gating and provide insights into cardiac disorder mechanisms.

Inhibition of tetrameric Patched1 by Sonic Hedgehog through an asymmetric paradigm

The paper reports the cryo-EM structure of tetrameric Ptch1 bound to ShhNp, revealing an asymmetric mechanism of Hh ligand inhibition.