Y. Fan

Defining ancestry, heritability and plasticity of cellular phenotypes in somatic evolution

The paper introduces PATH, a framework to quantify heritability and plasticity of cellular phenotypes in somatic evolution using single-cell data from mouse and human samples.

Ovarian disrupting effects and mechanisms of long- and short-chain per- and polyfluoroalkyl substances in mice

This study investigates the effects of long- and short-chain PFAS on ovarian function in mice, finding that long-chain PFAS disrupt follicle maturation and ovulation via PPARγ activation.

HCCDB v2.0: Decompose the Expression Variations by Single-cell RNA-seq and Spatial Transcriptomics in HCC

HCCDB v2.0 integrates bulk, single-cell, and spatial transcriptomic data from HCC samples to enhance meta-analysis and provide new metrics for gene expression patterns.

Timp2 loss-of-function mutation and TIMP2 treatment in murine model of NSCLC: modulation of immunosuppression and oncogenic signaling

TIMP2 treatment compensates for TIMP2 mutation to reveal immunomodulatory and oncogene regulation that inhibits tumor growth and metastatic niche formation.

DNA sensor ABCF1 phase separates with cccDNA to inhibit HBV replication

This study identifies ABCF1 as a host protein that interacts with cccDNA, forming phase-separated condensates to suppress HBV replication through innate immune activation and transcription inhibition.

Multifrequency control of Faraday wave bioassembly for constructing multiscale hPSC-derived neuronal networks

This paper introduces a multifrequency Faraday wave bioassembly technique to create multiscale neuronal networks from hPSCs, demonstrating spontaneous neuroelectrical activity.

Engineered immunomodulatory extracellular vesicles derived from epithelial cells acquire capacity for positive and negative T cell co-stimulation in cancer and autoimmunity

This study engineers extracellular vesicles from epithelial cells to express immunomodulatory proteins, showing they can induce both positive and negative T cell co-stimulation in cancer and autoimmunity.

Polygonum Cillinerve polysaccharide inhibits Transmissible gastroenteritis virus by regulating microRNA-181

This study shows that Polygonum Cillinerve polysaccharide inhibits TGEV infection by modulating miRNA-181 and reducing apoptosis in PK15 cells.

Analysis of uveal melanoma scRNA sequencing data identifies neoplastic-immune hybrid cells that exhibit metastatic potential

The paper identifies neoplastic-immune hybrid cells in uveal melanoma scRNA-seq data that exhibit metastatic potential through gene expression and ligand-receptor analysis.

BBQ methods: Streamlined workflows for Bacterial Burden Quantification in infected cells by confocal microscopy

This paper introduces two automated methods for quantifying bacterial burden in macrophages using confocal microscopy, validated with Salmonella and Mycobacterium models.

Pseudorabies virus causes splenic injury via inducing the oxidative stress and apoptpsos related factors in mice

This study shows PRV infection increases oxidative stress and apoptosis in mouse spleen, with elevated ROS and MDA levels and reduced antioxidant enzymes.

Genetically Encoded Boronolectin as a Specific Red Fluorescent UDP-GlcNAc Biosensor

This paper develops a genetically encoded red fluorescent UDP-GlcNAc biosensor using a boronolectin and validates its function in live cells.

Development, Characterization, and Structural Analysis of a Genetically Encoded Red Fluorescent Peroxynitrite Biosensor

The paper develops a red fluorescent peroxynitrite biosensor (pnRFP) using a genetically encoded boronic acid-containing amino acid, characterizes its properties, and elucidates its sensing mechanism through structural analysis.

BRD9-SMAD2/3 orchestrates stemness and tumorigenesis in pancreatic ductal adenocarcinoma

This study identifies BRD9 as a key regulator of pancreatic cancer stem cell stemness and tumorigenesis, showing that its inhibition reduces CSC self-renewal and enhances chemotherapy response.

Accurate and Rapid Detection of Peritoneal Metastasis from Gastric Cancer by AI-assisted Stimulated Raman Cytology

This paper develops an AI-assisted stimulated Raman cytology method to detect peritoneal metastasis in gastric cancer with high accuracy and speed.

A robotic system for automated genetic manipulation and analysis of Caenorhabditis elegans

This paper introduces a robotic system for automated genetic manipulation of C. elegans, demonstrating reliability and throughput comparable to manual methods.

A novel method revealing animal evolutionary relationships based on milk Mid-infrared

This study uses mid-infrared spectra of milk to establish models for assessing mammalian evolutionary relationships and genetic distances with high accuracy.

Flow-induced mechanical coupling between perinuclear actin cap and nucleus governs spatiotemporal regulation of YAP transport

This study investigates how flow-induced mechanical forces regulate YAP nucleocytoplasmic transport through actin cap formation and nuclear stiffness changes, revealing a unified mechanism linking mechanical stimulation to transcriptional regulation.

Genetic, Clinical Underpinnings of Brain Change Along Two Neuroanatomical Dimensions of Clinically-defined Alzheimer's Disease

This study identifies two brain atrophy dimensions in Alzheimer's disease using Surreal-GAN and links them to genetic and clinical factors, revealing distinct pathological mechanisms.

Creating an atlas of the bone microenvironment during oral inflammatory-related bone disease using single-cell profiling

This study creates a single-cell atlas of the bone microenvironment in healthy and inflammatory states, identifying key MSC subclusters and their biomarkers in apical periodontitis.

Multiscale functional connectivity patterns of the aging brain learned from rsfMRI data of 4,259 individuals of the multi-cohort iSTAGING study

The paper develops a brain age prediction model using multiscale functional connectivity from rsfMRI data of 4,259 individuals, showing that multiscale measures improve prediction and that brain age gaps correlate with cognitive and clinical measures.

Development of Equine Polyclonal Antibodies as a Broad-Spectrum Therapy Against SARS-CoV-2 Variants

This paper develops equine polyclonal antibodies that broadly neutralize major SARS-CoV-2 variants, showing high efficacy in vitro and ex vivo with low cost and potential for large-scale production.

The adipose-neural axis is critically involved in cardiac arrhythmias

This study shows that the adipose-neural axis contributes to cardiac arrhythmias through leptin-induced activation of sympathetic neurons and subsequent effects on cardiomyocytes.

A common mechanism of temperature-sensing in thermoTRP channels

This study identifies a common mechanism for temperature sensing in TRPM8 and TRPV1 thermoTRP channels through water-protein interaction changes.

Cell detoxification of secondary metabolites by P4-ATPase mediated vesicle transport

The paper identifies a P4-ATPase gene in a fungus that mediates detoxification of immunosuppressants via vesicle transport, with cross-species resistance applications in plants and human health.

ILC3s select for RORγt+ Tregs and establish tolerance to intestinal microbiota

This study identifies ILC3s as key regulators that promote RORγt+ Tregs and suppress Th17 cells to maintain intestinal tolerance to microbiota.

piggyBac-Mediated Genomic Integration of Linear dsDNA-Based Library for Deep Mutational Scanning in Mammalian Cells

This paper develops two new strategies for constructing large variant DMS libraries in mammalian cells using piggyBac-mediated genomic integration and in-vitro ligation.

Combined SHP2 and ERK inhibition for the treatment of KRAS-driven Pancreatic Ductal Adenocarcinoma

This paper investigates the combination of SHP2 and ERK inhibition as a treatment for KRAS-driven pancreatic ductal adenocarcinoma, showing synergistic anticancer effects and tumor regression in mouse models.

A magnetic field prevents alcoholic liver disease by reducing oxidative stress

This study shows that a specific static magnetic field reduces oxidative stress and liver damage caused by alcohol consumption in mice.

IGF1 Signaling in Temporomandibular Joint Fibrocartilage Stem Cells Regulates Cartilage Growth and Homeostasis in Mice

This study shows that Igf1 deletion in TMJ fibrocartilage stem cells causes cartilage degeneration and worsens osteoarthritis in mice.