W. Dai

Parallel Analyses by Mass Spectrometry (MS) and Reverse Phase Protein Array (RPPA) Reveal Complementary Proteomic Profiles in Triple-Negative Breast Cancer (TNBC) Patient Tissues and Cell Cultures

The paper compares Mass Spectrometry (MS) and Reverse Phase Protein Array (RPPA) in TNBC samples to assess their performance and identify complementary proteomic profiles.

Structural and Biophysical Dynamics of Fungal Plasma Membrane Proteins and Implications for Echinocandin Action in Candida glabrata

This study investigates the structural and dynamic organization of plasma membrane proteins in Candida glabrata using cryo-ET and live cell imaging, revealing how echinocandin drugs affect their distribution and function.

Targeting oncogenic K-Ras mutants with a small-molecule degrader through Nedd4-1

This study develops a small-molecule degrader, XMU-MP-9, that promotes ubiquitination and degradation of K-Ras mutants, including K-RasG12V, by enhancing Nedd4-1 interaction with K-Ras.

ΔNp63-restricted viral mimicry response impedes cancer cell viability and remodels tumor microenvironment in esophageal squamous cell carcinoma

This study identifies ΔNp63's role in restricting viral mimicry response in ESCC, reducing cell viability, and modulating tumor microenvironment immune cells.

Plk3 Regulates Bacteremia and Supports Sepsis

This study shows that Plk3 promotes bacterial spread and sepsis by enabling bacteremia, as Plk3-deficient mice survive sepsis models with reduced bacterial load in blood.

Revealing nanoscale structure and interfaces of protein and polymer condensates via cryo-electron microscopy

This study uses cryo-EM to analyze nanoscale structures of protein and polymer condensates, revealing core-shell morphologies and internal inhomogeneities.

Cell type-specific translational regulation by human DUS enzymes

This study identifies DUS enzymes' role in tRNA modification and shows cell type-specific effects on translation.

Oxidized LDL regulates efferocytosis through the CD36-PKM2-mtROS pathway

This study identifies the CD36-PKM2-mtROS pathway as a mechanism by which oxidized LDL disrupts efferocytosis, contributing to atherosclerosis progression.

Tip60-mediated Rheb acetylation links palmitic acid with mTORC1 activation and insulin resistance

This study shows that palmitic acid induces mTORC1 activation via Tip60-mediated Rheb acetylation, leading to insulin resistance, while unsaturated fatty acids do not.

Hypoxia Bi-directionally Regulates Gut Vascular Barrier through HIF-1α-dependent Mechanism in vitro

Use of Artificial Intelligence for Automated Detection and Surveillance of Red Imported Fire Ants Nests

This study develops an AI system using robotic dogs to detect red imported fire ant nests with 95% precision, improving surveillance and pesticide delivery in open fields.

Rescue of proteotoxic stress and neurodegeneration by the Zn2+ transporter ZIP7

This study shows ZIP7 enhances ERAD by supplying cytosolic Zn2+ for Rpn11 deubiquitination, rescuing vision loss in Drosophila with misfolded rhodopsin.

FASN-deficiency induces a cytosol-to-mitochondria citrate flux to mitigate detachment-induced oxidative stress

This study shows that FASN-deficiency leads to a cytosol-to-mitochondria citrate flux via IDH1 and CTP, which helps cancer cells resist oxidative stress during detachment.

PilD mutant is a new cheater in Pseudomonas aeruginosa quorum-sensing evolution

The paper identifies a new cheater mutant, PilD, that evolves from a LasR-MexT mutant in Pseudomonas aeruginosa through genome sequencing and proteolytic phenotype analysis.

Gene Gain and Loss from the Asian Corn Borer W Chromosome

The paper presents a chromosome-level genome assembly of the Asian corn borer, revealing the W chromosome's unique features, including gene gain, pseudogenization, and enrichment for DNA-related functions.

When two is a crowd - Structural mapping of PEAK pseudokinase interactions identifies 14 3 3 as a molecular switch for PEAK3/Crk signaling.

The paper identifies 14-3-3 as a molecular switch for PEAK3/Crk signaling by mapping PEAK pseudokinase interactions and demonstrating a conserved high affinity 14-3-3 motif.

Discovery of RNA polymerase α-subunit protein as a novel quorum sensing reprograming factor in Pseudomonas aeruginosa

The paper identifies RpoA, an RNA polymerase α-subunit, as a novel factor enabling quorum sensing reprogramming in Pseudomonas aeruginosa LasR-null mutants.

Sensory convergence in the world s largest cavefish diversification: patterns of neuromast evolution, distribution and associated behaviour in Sinocyclocheilus

This study examines neuromast evolution and distribution in Sinocyclocheilus cavefish, finding asymmetry and convergence in cave-adapted species with distinct wall-following behaviours.

Collective border cell migration requires the zinc transporter Catsup to limit endoplasmic reticulum stress

The paper identifies Catsup as essential for border cell migration by preventing ER stress and shows that zinc transport is critical for this process.

Olanzapine leads to nonalcoholic fatty liver disease through the apolipoprotein A5 pathway

This study shows olanzapine induces NAFLD via the apoA5 pathway by increasing hepatic apoA5 protein and sortilin levels, with APOA5 knockdown reversing steatosis.

The component parts of bacteriophage virions accurately defined by a machine-learning approach built on evolutionary features.

This paper develops a machine learning tool called STEP3 to accurately define bacteriophage virion components using evolutionary features, validated with mass spectrometry.

Immunogenicity of an AAV-based, room-temperature stable, single dose COVID-19 vaccine in mice and non-human primates

This paper evaluates an AAV-based COVID-19 vaccine in mice and non-human primates, showing sustained neutralizing antibodies and memory T-cells, with room-temperature stability and scalable production.

Structure-Based Design, Synthesis and Biological Evaluation of Peptidomimetic Aldehydes as a Novel Series of Antiviral Drug Candidates Targeting the SARS-CoV-2 Main Protease

This study designs, synthesizes, and evaluates two peptidomimetic aldehydes as antiviral drug candidates targeting SARS-CoV-2 main protease, showing potent inhibition and anti-infection activity.

CRISPR-assisted targeted enrichment-sequencing (CATE-seq)

This paper introduces CATE-seq, a CRISPR-based targeted sequencing method that outperforms hybridization-based techniques in simplicity, specificity, sensitivity, throughput, and scalability.

Stimulus-responsive self-assembly of protein-based fractals by computational design

This paper designs stimulus-responsive protein-based fractals using computational methods, achieving self-similar structures across multiple length scales.

Cancer gene therapy by NF-κB-activated cancer cell-specific expression of CRISPR/Cas9 targeting to telomere

This paper demonstrates a gene therapy approach using NF-κB-activated CRISPR/Cas9 to specifically target and kill cancer cells without affecting normal cells.

Gene activation by a CRISPR-assistant trans enhancer

This study develops a CRISPR-assistant trans enhancer to efficiently activate gene expression by combining dCas9-VP64/sgRNA with a CMV enhancer.

Cancer therapy with a CRISPR-assistant telomerase-activating gene expression system

The paper develops a gene therapy system that uses telomerase activity to kill cancer cells in mice without affecting normal cells.