J. Sheng

The stability of lower genital tract microbiota correlates with reproductive system function and in vitro fertilization and frozen embryo transfer outcomes in women with polycystic ovarian syndrome

This study investigates the relationship between lower genital tract microbiota and reproductive outcomes in PCOS patients, finding that higher Lactobacillus abundance correlates with better IVF-FET outcomes.

Immunosuppressive tumor microenvironment of osteosarcoma

This study identifies immunosuppressive mechanisms in osteosarcoma using single-cell and spatial transcriptomics, revealing interactions between MDSCs, T-cells, and tumor cells that promote an immunosuppressive microenvironment.

Decreased fucosylation impacts epithelial integrity and increases risk for COPD.

This study shows decreased fucosylation impairs epithelial integrity and increases COPD risk by affecting E-cadherin function and cell differentiation.

Ellagic acid ameliorates atherosclerosis by targeting the epidermal growth factor receptor

This study shows ellagic acid reduces atherosclerosis by binding EGFR, activating the EGFR-ERK pathway, stabilizing LDLR mRNA, and increasing LDLR protein levels in mice.

Targeting Ribosome Biogenesis as a Novel Therapeutic Approach to Overcome EMT-related Chemoresistance in Breast Cancer.

This study identifies ribosome biogenesis as a key driver of EMT/MET transitions in breast cancer and shows that targeting this pathway enhances chemotherapy efficacy against chemoresistant tumors.

High-throughput tagging of endogenous loci for rapid characterization of protein function

This paper introduces HITAG, a method for high-throughput tagging of endogenous loci to study protein function, and demonstrates its use in analyzing stress granule-associated proteins.

Bioorthogonal in-cell Labeling and Profiling of N6-isopentenyladenosine (i6A) Modified RNA

The paper introduces a method to detect and analyze i6A-modified RNA with single-base resolution using iodine-mediated oxidation and reverse transcription.

The Functional Landscape of SARS-CoV-2 3CL Protease

This study maps the functional landscape of SARS-CoV-2 3CL protease by profiling all single mutants and identifies resistance mutations to nirmatrelvir.

Bio-orthogonal chemistry-based conjugation strategy facilitates investigation of impacts of s2U, s4U, m1A and m6A guide RNA modifications on CRISPR activity.

The paper develops a bio-orthogonal conjugation strategy to efficiently assemble modified sgRNAs and investigates their impact on CRISPR activity.

Long-term ovarian cancer survivors: spatial transcriptomics depict ligand-receptor crosstalk heterogeneity at the tumor-stroma interface

Nature-Inspired Chemical Probes for in-cell Labeling of Lipidized RNA and Identification of New Regulatory Enzymes

The paper develops chemical labeling strategies to tag lipid-modified RNAs and identify new regulatory enzymes involved in prenylation.

Human endogenous retrovirus activation contributes to biliary atresia pathogenesis through re-education of resident macrophages

This study identifies HERV activation in BA livers and shows it re-educates macrophages, impairing neutrophil recruitment and causing liver damage, suggesting therapeutic targets.

The bladder microbiome, metabolome, cytokines, and phenotypes in patients with systemic lupus erythematosus

This study examines the bladder microbiome, metabolome, and cytokines in SLE patients, finding associations between microbial composition and disease-related metabolites and cytokines.

Bisphenol S and bisphenol F are less disruptive to cardiac electrophysiology and potentially safer for use in medical products, as compared to bisphenol A

This study compares the effects of bisphenol analogues on cardiac electrophysiology, finding BPA and BPF inhibit ion channels more than BPS.

Fate mapping analysis reveals a novel dermal migratory Langerhans-like cell population

The paper identifies a novel dermal migratory Langerhans-like cell population using fate mapping and single-cell RNA sequencing, showing distinct migration and replenishment characteristics compared to Langerhans cells.

Comparative transcriptome analysis reveals the intensive early-stage responses of host cells to SARS-CoV-2 infection

This study compares the early host responses to SARS-CoV-2, SARS-CoV, and MERS-CoV infections using transcriptome analysis, revealing distinct patterns of cytokine and antiviral gene expression.

Bio-Orthogonal Chemistry Enables Solid Phase Synthesis of Long RNA Oligonucleotides

This paper develops a bio-orthogonal chemistry method for synthesizing long RNA oligonucleotides, achieving 76-nt tRNA and 101-nt sgRNA with high purity and functional fidelity.

The role of cation diffusion facilitator CDF-1 in lipid metabolism in Caenorhabditis elegans

This study identifies CDF-1 and SUR-7 as regulators of lipid metabolism in C. elegans, linking zinc homeostasis to lipid accumulation via the SREBP-SCD pathway.

A chemiluminescent protease probe for rapid, sensitive, and inexpensive detection of live Mycobacterium tuberculosis

A chemiluminescent probe detects live Mycobacterium tuberculosis with high sensitivity and speed, enabling improved TB diagnostics and drug resistance monitoring.

Base Pairing and Functional Insights into N3-methylcytidine (m3C) in RNA

This study investigates the effects of N3-methylcytidine (m3C) modification on RNA base pairing and reverse transcription, showing its impact on stability, discrimination, and mutation induction.

Transcriptome profiling reveals the mechanism of ripening and epidermal senescence in passionfruit (Passiflora edulia Sims)

This study identifies gene expression changes and pathways involved in passion fruit ripening and senescence using RNA sequencing and validates findings with RT-qPCR.

Biodegradation of polyester polyurethane by Aspergillus flavus G10

This study investigates the biodegradation of polyester polyurethane by Aspergillus flavus G10 using multiple analytical techniques and identifies conditions that enhance degradation.

Abortion and various associated risk factors in dairy cow and sheep in Ili, China

This study investigates abortion rates and risk factors in dairy cows and sheep in Ili, China, identifying Brucella melitensis as the main pathogen and highlighting management factors contributing to reproductive issues.

Cryo-EM structure of C9ORF72-SMCR8-WDR41 reveals the role as a GAP for Rab8a and Rab11a

This paper reports the cryo-EM structure of the C9ORF72-SMCR8-WDR41 complex, revealing its role as a GAP for Rab8a and Rab11a.

Single-cell RNA-seq reveals endoimmune cells in zebrafish

The paper identifies endoimmune cells, a novel subpopulation of endothelial cells in zebrafish, using single-cell RNA sequencing and shows their involvement in immune responses.

Extensive genetic diversity of bat-borne polyomaviruses reveals inter-family host-switching events

This study identifies extensive genetic diversity of bat-borne polyomaviruses and reports three inter-family host-switching events, challenging the notion of strict host restriction in mammalian PyVs.

Molecular basis for the acid initiated uncoating of human enterovirus D68

This study determines the structural changes in EV-D68 under acidic conditions, revealing the molecular mechanism of uncoating and acid lability.