F. Sun

FAST-STEM: A human pluripotent stem cell engineering toolkit for rapid design-build-test-learn development of human cell-based therapeutic devices

FAST-STEM accelerates human pluripotent stem cell engineering by enabling rapid transgene integration and differentiation into multiple cell types.

Exo70 promotes herpesvirus secretory vesicle tethering and virion release in an exocyst complex-dependent manner

This study identifies Exo70's role in herpesvirus secretory vesicle tethering and virion release via the exocyst complex, showing ES2 inhibits herpesvirus infection.

Discovery of Alkenyl Oxindole as a Novel PROTAC Moiety for Targeted Protein Degradation via CRL4DCAF11 Recruitment

The paper develops JQ1-alkenyl oxindole conjugates that degrade BRD4 via the ubiquitin-proteasome system and recruits CRL4DCAF11, identifying HL435 as a potent antitumor lead.

Structural elucidation of how ARF small GTPases induce membrane tubulation for vesicle fission

The paper determines the structure of ARF6 protein lattice on tubulated membranes, revealing its role in vesicle fission and how GAP proteins interact with it.

Parallel Molecular Data Storage by Printing Epigenetic Bits on DNA

This paper introduces a DNA-based data storage method using epigenetic modifications, achieving scalable and parallel data writing and retrieval.

Xanthomonas euvesicatoria effector XeAvrRxo1 triggers a Rxo1-mediated defense response in Nicotiana benthamiana and its chaperone Xe4429 functions as an antitoxin, an expression repressor, and an enhancer of XeAvrRxo1 secretion

This study identifies XeAvrRxo1 as a virulence and avirulence effector in X. euvesicatoria and shows that its chaperone Xe4429 suppresses toxicity, represses expression, and enhances secretion of XeAvrRxo1.

IsoVEM: Isotropic Reconstruction for Volume Electron Microscopy Based on Transformer

The paper introduces IsoVEM, a transformer-based algorithm for isotropic reconstruction in volume electron microscopy, improving axial resolution and repairing damaged sections.

Cryo-EM structures of human SID-1 transmembrane family proteins and implications for their low-pH-dependent RNA transport activity

The paper presents cryo-EM structures of human SIDT1 and SIDT2, revealing their dimeric architecture and demonstrating low-pH-dependent RNA binding and oligomerization.

Multiscale mechanisms driving tissue rupture by invading cells

This study identifies a mechanism where invading cells and tissue barriers coordinate to rupture barriers through intercellular adhesions and subcellular contractility, challenging existing models of collective invasion.

In situ structural insights into the excitation contraction coupling mechanism of skeletal muscle

This study reveals the nanometre-resolution structure of the RyR1-DHPR supercomplex in skeletal muscle triads, showing their unique arrangement and physical coupling mechanism for excitation-contraction coupling.

Longer mandible or nose? Co-evolution of feeding organs in early elephantiforms

This study investigates the co-evolution of feeding organs in early elephantiforms, finding that feeding function shifted from the mandible to the trunk, enabling proboscideans to expand into new ecological niches.

Improving PD-1 blockade plus chemotherapy for complete remission of lung cancer by nanoPDLIM2

This study develops nanoPDLIM2, a PDLIM2-expressing nanoparticle, which enhances PD-1 blockade and chemotherapy efficacy in lung cancer by reactivating tumor immunity and reducing drug resistance.

EHMT2/G9a-Inhibition Reprograms Cancer-Associated Fibroblasts (CAFs) to a More Differentiated, Less Proliferative and Invasive State

This study identifies EHMT2 as a key regulator of CAF hyperproliferation and pro-invasive behavior, showing its inhibition reprograms CAFs to a less aggressive state.

CytoSIP: An annotated structural atlas for interactions involving cytokine or cytokine receptor

CytoSIP provides a database of cytokine-cytokine receptor interactions with SNPs, interfaces, and disease associations to aid drug design and precision medicine.

In-cell structural insight into the stability of sperm microtubule doublet

The paper uses cryo-electron tomography to determine the in-cell structure of mouse sperm microtubule doublets, revealing their molecular architecture and stability mechanisms.

A novel mouse allele of the DNA/RNA helicase senataxin (Setxspcar3) causing meiotic arrest of spermatocytes and male infertility

This study identifies a new Setx allele causing male infertility and female subfertility in mice by disrupting meiosis and spermatogenesis through R-loop accumulation and DNA damage.

COMT inhibitor tolcapone represses the migration and invasion of trophoblast and results in preeclampsia-like phenotypes in mice

This study shows that tolcapone causes embryo lethality and preeclampsia-like phenotypes in mice by inhibiting trophoblast migration and invasion.

Exploring the genome and protein space of viruses

The paper estimates the genetic diversity of viruses using simulated discovery processes, finding that less than 3% of viral genetic diversity has been uncovered.

HOPE-SIM, a cryo-structured illumination fluorescence microscopy system for accurately targeted cryo-electron tomography

The paper introduces HOPE-SIM, a cryo-CLEM system that improves targeting precision for cryo-ET by integrating 3D SIM and software, achieving 150 nm correlation accuracy.

ELI trifocal microscope: A precise cryogenic fabrication system to prepare target cryo-lamellae of cells for in situ cryo-ET study

The paper introduces ELI-TriScope, a cryogenic system for precise cryo-lamella preparation, achieving 100% success in targeting HeLa cell centrosomes for cryo-ET structural analysis.

Formation of secondary allo-bile acids by novel enzymes from gut Firmicutes

This paper identifies novel enzymes from gut Firmicutes that convert 'kinked' primary bile acids into 'flat' secondary bile acids, with increased abundance in colorectal cancer patients.

Old Age Drives Fusion and Expansion of Vitellogenin Vesicles in the Intestine of Caenorhabditis elegans

This study examines age-related changes in vitellogenin vesicles and yolk organelles in C. elegans, finding significant expansion and fusion of these structures in post-reproductive worms.

Immuno-electron microscopy localizes Caenorhabditis elegans vitellogenins along the classic exocytosis route

This study uses immuno-electron microscopy to map the subcellular localization of vitellogenins in C. elegans, showing they follow the classic exocytic pathway.

Structure of maize BZR1-type β-amylase BAM8 provides new insights into its noncatalytic adaptation

The paper determines the crystal structure of maize BAM8, a noncatalytic BZR1-type β-amylase, and reveals its structural adaptations and potential regulatory roles.

Introducing an argonaute-facilitated PCR platform

This paper introduces an argonaute-facilitated PCR platform that improves PCR sensitivity by using MejAgo to enhance guide DNA/template pairing, eliminating the need for an annealing step.

A novel cryo-electron microscopy support film based on 2D crystal of HFBI protein

This paper introduces a new cryo-EM support film using HFBI protein 2D crystals to improve protein structure resolution by reducing preferred orientation and denaturation during vitrification.

Molecular basis for the R-type anion channel QUAC1 activity in guard cells

This study determines the cryo-EM structure of QUAC1, an R-type anion channel, revealing its dimeric architecture and mechanism of malate-mediated activation.

Imaging biological samples by integrated differential phase contrast (iDPC) STEM technique

This study demonstrates that iDPC-STEM provides superior contrast and structural detail in biological samples compared to conventional TEM, especially at low doses and for thick sections.

Maternal RND3/RhoE deficiency impairs placental mitochondrial function in preeclampsia by modulating PPARγ-UCP2 cascade

This study identifies Rnd3 as a protective factor in placental mitochondria through PPARγ-UCP2 signaling, showing its role in mitigating oxidative stress in preeclampsia.

Metabolic Complementation between Glucose and Amino Acid Drives Hepatic De Novo Lipogenesis and Steatosis

The paper identifies glucose and amino acid contributions to hepatic de novo lipogenesis and steatosis in obesity using metabolic tracing and in vivo studies.