J. Fang

Substrate specificity and protein stability drive the divergence of plant-specific DNA methyltransferases

This study investigates how CMT2 and CMT3 diverge in substrate specificity and protein stability, revealing evolutionary mechanisms of DNA methylation in plants.

Universal protection of allogeneic cell therapies from natural killer cells via CD300a agonism

This paper develops CD300a TASR, an engineered ligand that universally protects HLA-deficient allogeneic T cells from NK cell rejection and enhances CAR-T efficacy.

RBM39 degrader invigorates natural killer cells to eradicate neuroblastoma despite cancer cell plasticity

This study shows that targeting RBM39 with indisulam promotes natural killer cell activity and eradicates neuroblastoma regardless of cellular state plasticity.

Conditional c-MYC activation in catecholaminergic cells drives distinct neuroendocrine tumors: neuroblastoma vs somatostatinoma

This study creates two mouse models with conditional c-MYC activation in specific cell types, leading to distinct neuroendocrine tumors resembling human neuroblastoma and somatostatinoma.

A Microphysiological HHT-on-a-Chip Platform Recapitulates Patient Vascular Lesions

This paper develops a microphysiological HHT-on-a-chip platform that recapitulates patient vascular lesions and demonstrates sensitivity to pazopanib, a drug effective in HHT patients.

Disrupting CD38-driven T cell dysfunction restores sensitivity to cancer immunotherapy

This study shows that targeting CD38 improves T cell function and restores response to immunotherapy in melanoma models.

Genome assembly of the rare and endangered Grantham's camellia, Camellia granthamiana

This paper presents a chromosome-scale genome assembly of the rare Camellia granthamiana using PacBio and Omni-C data, providing a resource for conservation and phylogenetic studies.

The PCM scaffold enables RNA localization to centrosomes

The paper investigates how Plp mRNA localizes to centrosomes, showing that its coding sequence is necessary and sufficient for localization and that PCM scaffold interactions facilitate this process.

Discrimination of Isomeric Glycans via High-sensitive Engineered Nanopore

This paper designs an engineered nanopore to distinguish isomeric glycans with high sensitivity and achieves over 90% separation accuracy without machine learning.

Comparative analysis of methodologies for detecting extrachromosomal circular DNA

This study compares 7 analysis pipelines and 7 experimental methods for detecting extrachromosomal circular DNA, identifying the most effective approaches for short-read and long-read sequencing data.

Structural basis for the allosteric regulation and dynamic assembly of DNMT3B

This study determines the cryo-EM structure of DNMT3B under various oligomerization states, revealing how its domains interact to regulate its assembly and function.

Expansion Strategy-Driven Micron-Level Resolution Mass Spectrometry Imaging of Lipids in Mouse Brain Tissue

This paper introduces a method to enhance spatial resolution in lipid mass spectrometry imaging of mouse brain tissue by using a swellable polyelectrolyte gel, achieving subcellular-level visualization.

Distinct Microstructural Heterogeneities Underpin Specific Micromechanical Properties in Human ACL Femoral and Tibial Entheses

This study identifies distinct microstructural and biomolecular features in ACL femoral and tibial entheses that contribute to their specific mechanical properties.

Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling

This study functionally analyzes Cdc20's role in mitotic checkpoint signaling by identifying the CRY box's critical role in SAC through MCC formation and APC/C interaction.

Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing is associated with therapeutic response to splicing inhibitor

This study shows JMJD6 links pre-mRNA splicing and metabolism in MYC-driven cancers, and its inhibition enhances therapeutic response to splicing inhibitors.

Resident and recruited macrophages differentially contribute to cardiac healing after myocardial ischemia

This study shows resident macrophages improve cardiac remodelling while recruited macrophages influence infarct size after myocardial ischemia/reperfusion injury.

A CRISPR-drug perturbational map for identifying new compounds to combine with commonly used chemotherapeutics.

The paper uses CRISPR knockout screens to identify drug combinations that enhance the effectiveness of common chemotherapeutics in neuroblastoma cells.

Augmentation of Histone Deacetylase 6 Activity Impairs Mitochondrial Respiratory Complex I in Ischemic/Reperfused Diabetic Hearts

This study shows that increasing HDAC6 activity impairs mitochondrial complex I in diabetic hearts subjected to ischemia/reperfusion, and that inhibiting HDAC6 improves cardiac function.

Spatial and functional arrangement of Ebola virus polymerase inside phase-separated viral factories

This study investigates the spatial and functional organization of Ebola virus polymerase within viral factories, revealing distinct structural forms and their roles in viral replication.

Monkeypox virus quadrivalent mRNA vaccine induces antibody responses and cellular immunity and protects mice against Vaccinia virus

This study develops two quadrivalent mRNA vaccines against monkeypox virus and shows they induce antibodies, cellular immunity, and protection against Vaccinia virus in mice.

Single-cell Landscape Analysis Unravels Molecular Programming of the Human B Cell Compartment in Chronic GVHD

This study uses scRNA-Seq to analyze B cells from allo-HCT patients, revealing molecular differences in B cell subsets associated with active versus non-active cGVHD.

In silico labeling enables kinetic myelination assay in brightfield

This paper develops an in-silico labeling method using a generative adversarial network to predict fluorescent images from brightfield images, enabling kinetic myelination assays.

Csf1 from marrow adipogenic precursors is required for osteoclast formation and hematopoiesis in bone

Proximity interactome analysis of Lassa polymerase reveals eRF3a/GSPT1 as a druggable target for host directed antivirals.

The paper identifies eRF3a/GSPT1 as a druggable host target for antiviral therapy against Lassa virus by analyzing its interactome using proximity proteomics.

Separation of phosphatase and kinase activity within the Bub complex is required for proper mitosis

This study shows that separating kinase and phosphatase activities in the Bub complex is essential for proper mitosis, as merging these functions leads to mitotic defects.

CV-containing vesicle budding from chloroplast inner envelope membrane is mediated by clathrin but inhibited by GAPC

The paper shows that clathrin facilitates CV-containing vesicle budding from chloroplasts, while GAPC inhibits this process and affects stress responses.

RNA-binding protein Orb2 causes microcephaly and supports centrosome asymmetry in Drosophila neural stem cells

This study shows Orb2 supports centrosome asymmetry in Drosophila NSCs, leading to microcephaly when lost, and identifies a conserved role for CPEB proteins in centrosome regulation.

Orb-dependent polyadenylation contributes to PLP expression and centrosome scaffold assembly

This study shows Orb regulates PLP expression through polyadenylation, affecting centrosome function and embryonic viability.

Functional interactomes of the Ebola virus polymerase identified by proximity proteomics in the context of viral replication

The paper identifies functional interactors of the Ebola virus polymerase using proximity proteomics and validates their role in promoting viral replication.

Revealing the full biosphere structure and versatile metabolic functions in the deepest ocean sediment of the Challenger Deep

This study uses deep metagenomics to explore microbial communities in Challenger Deep sediment, revealing new MAGs, metabolic functions, and virus-host interactions in hadal zone ecosystems.