M. Luo

Dynamic Regulation of Innate Lymphoid Cell Development During Ontogeny

This study investigates the developmental changes in innate lymphoid cell (ILC) subsets using scRNA-seq and differentiation assays, revealing distinct regulatory programs in fetal and adult stages.

Equip Fusobacterium nucleatum genetic tool kits with compatible shuttle vectors and engineered intermediatory E. coli strains for enhanced transformation efficiency

This paper develops genetic tools for Fusobacterium nucleatum by engineering E. coli intermediates and shuttle vectors, achieving 5000 transformants per microgram plasmid.

Enhancing reproducibility and decentralization in single cell research with biocytometry

This study evaluates biocytometry's performance in a decentralized setting, showing its ability to accurately count rare cells across multiple labs with minimal training.

Perfringolysin O pore-forming complexes are predominantly integral multiples of six subunits

The paper investigates the structural composition of Perfringolysin O (PFO) complexes, finding that they are predominantly hexameric and built from hexameric sub-complexes.

Epigenetic regulation of alveolar macrophage homeostasis by histone deacetylase 1 and 2

This study shows HDAC1 and HDAC2 are essential for alveolar macrophage homeostasis by suppressing Bim expression and co-regulating differentiation with LSD1.

Single-nucleus Multiomic Analyses Identifies Gene Regulatory Dynamics of Phenotypic Modulation in Human Aneurysmal Aortic Root

This study uses single-nucleus multiomic and spatial transcriptomic data to identify gene regulatory dynamics and key regulators in human aortic root tissue under healthy and Marfan syndrome conditions.

Large-scale multi-omics analyses identified root-microbiome associations underlying plant nitrogen nutrition

The paper identifies root-microbiome associations affecting plant nitrogen nutrition through multi-omics analyses of 1,341 rapeseed samples.

Structural insights into peptidoglycan hydrolysis by the FtsEX system in Escherichia coli during cell division

This study presents structural and biochemical analysis of E. coli FtsEX complex, revealing ATP's role in stabilization and EnvC-mediated activation of PG hydrolysis.

Berberine impairs spermatogenesis in mice by modulating ornithine metabolism via gut microbiota regulation

This study shows berberine reduces sperm count and testosterone in mice by altering gut microbiota, specifically reducing Muribaculaceae, and affecting ornithine metabolism.

HaloTag display enables quantitative single-particle characterization and functionalization of engineered extracellular vesicles

The paper develops a HaloTag-based system for quantitatively characterizing engineered proteins on extracellular vesicles and compares it to antibody labeling.

Combining environmental DNA and remote sensing for efficient, fine-scale mapping of arthropod biodiversity

The paper combines environmental DNA and remote sensing to map arthropod biodiversity at fine spatial scales, revealing patterns in species richness and conservation value.

Hsa_circ_0005397 could promote hepatocellular carcinoma progression and metastasis through EIF4A3

This study identifies hsa_circ_0005397 as a promoter of hepatocellular carcinoma progression and metastasis via EIF4A3, showing its diagnostic and prognostic significance.

The Microspherule protein 1 (MCRS1) homolog interacts with the Myb-like transcription factor DRMY1 and is essential for embryogenesis in Arabidopsis thaliana

This study shows that MCRS1 interacts with DRMY1 and is essential for embryogenesis in Arabidopsis, but not for gametophyte mitosis.

Manipulation of Bacterial ROS Production Leads to Self-escalating DNA Damage and Resistance-resistant Lethality for Intracellular Mycobacteria

This study develops a resistance-resistant antimicrobial by inducing catastrophic DNA damage in intracellular mycobacteria through a hybrid organoruthenium-natural product that manipulates bacterial ROS production.

Enzyme-mediated alkynylation enables transcriptome-wide identification of pseudouridine modifications

The paper introduces ELAP-seq, a method to detect pseudouridine modifications in RNA with high sensitivity and resolution, identifying over 10,000 sites in human transcripts.

Pooled effector library screening in protoplasts rapidly identifies novel Avr genes

This paper develops a pooled effector library screening platform in plant protoplasts to identify Avr genes interacting with R genes, validating it by discovering known and novel Avr genes from wheat stem rust.

Metal-driven anaerobic oxidation of methane as an important methane sink in methanic cold seep sediments

The paper investigates metal-driven anaerobic methane oxidation in cold seep sediments, finding that Fe-AOM and Mn-AOM contribute ~3% to methane removal.

ABI5-FLZ13 Module Transcriptionally Represses Growth-related Genes to Delay Seed Germination in Response to ABA

This study identifies FLZ13 as an ABI5-interacting protein that co-represses growth-related genes to delay seed germination in response to ABA.

Proteomic Landscape of Human Spermatozoa: Optimized Extraction Method and Application

This study optimizes protein extraction methods for human sperm and investigates antibiotic effects on sperm proteome, finding increased concentration and malformed sperm with altered proteome expression.

The velvet family proteins mediate low resistance to isoprothiolane in Magnaporthe oryzae

This study identifies MoVelB, a velvet family transcription factor, as a determinant of low IPT resistance in M. oryzae by deforming the LaeA-VeA-VelB heterotrimer and down-regulating secondary metabolism and CYP450 genes.

Integrated analysis reveals FOXA1 and Ku70/Ku80 as direct targets of ivermectin in prostate cancer

This study identifies FOXA1 and Ku70/Ku80 as direct targets of ivermectin in prostate cancer, showing it induces cell cycle arrest, apoptosis, and synthetic lethality through chromatin accessibility reduction and DNA repair impairment.

A 4-bp natural deletion of maize Na+/H+ exchanger gene alters maize salt stress tolerance

This paper identifies a 4-bp deletion in the maize ZmSOS1 gene that causes salt sensitivity and develops a molecular marker to improve salt tolerance in breeding programs.

Zinc Finger MYND-Type Containing 8 (ZMYND8) is epigenetically regulated in mutant Isocitrate Dehydrogenase 1 (IDH1) glioma to promote radioresistance

This paper identifies ZMYND8 as a gene epigenetically regulated by mIDH1 that promotes radioresistance in glioma cells, and shows its knockdown enhances radiosensitivity.

Molecular mechanism of phospholipid transport at the bacterial outer membrane interface

This paper characterizes the MlaA-MlaC complex structure and mechanism for phospholipid transport across the bacterial outer membrane.

Chemical genetic screening identifies nalacin as an inhibitor of GH3 amido synthetase for auxin conjugation

The paper identifies nalacin as an inhibitor of GH3 amido synthetase, which regulates auxin conjugation in plants, with potential applications in agriculture and research.

Ratiometric NAD+ sensors reveal subcellular NAD+ modulators

The paper introduces NAD+ sensors to measure subcellular and clinical NAD+ dynamics, revealing NAD+ redistribution effects of -GPC in live cells.

Atomic model of Vesicular Stomatitis Virus and Mechanism of Assembly

The paper determines the in situ atomic structures of VSV components using cryoEM and sub-particle reconstruction, revealing their assembly mechanism through interactions.

Distinct subpopulations of DN1 thymocytes exhibit preferential γδ T lineage potential

This study identifies distinct DN1 thymocyte subpopulations with preferential γδ T lineage potential using scRNA-seq in mice.

Heterotrimeric G protein α subunit (GPA1) regulates the response to low-nitrogen stress in Arabidopsis by interacting with AtNRT1.4 and AtATG8a

AtGPA1 regulates low-nitrogen stress response in Arabidopsis by interacting with AtNRT1.4 and AtATG8a, affecting nitrate uptake and autophagy.

Expansion and Adaptive Evolution of the mTERF Gene Family in Plants

The paper analyzes the expansion and adaptive evolution of mTERF genes in plants, finding that M-class mTERF genes expanded via tandem duplication and evolved rapidly with positive selection in nucleic acid-binding sites.