X. Fang

Spatial organization of PI3K-PI(3,4,5)P3-AKT signaling by focal adhesions

This study identifies focal adhesions as the subcellular hubs organizing PI3K-PI(3,4,5)P3-AKT signaling, showing localized lipid production and AKT activation, and demonstrates that combined inhibition of PI3K and FAK enhances cancer cell suppression.

The genomic and epigenomic dynamics of hemizygous genes across crops with contrasting mating systems

This study examines hemizygous genes in 22 crop genomes, finding higher hemizygosity in clonal lineages and distinct expression and epigenetic features compared to diploid genes.

Deep learning based genomic breeding of pest-resistant grapevine

This study uses deep learning and genome-wide association studies to identify pest-resistant genes in grapevine, achieving high accuracy in predicting pest damage.

A CRISPR-Nonhomologous End-Joining-based strategy for rapid and efficient gene disruption in Mycobacterium abscessus

This paper introduces a CRISPR-NHEJ strategy for efficient gene disruption in Mycobacterium abscessus and shows that homologous recombination and single-strand annealing components are required for NHEJ repair.

Ligand supplementation restores the cancer therapy efficacy of an antirheumatic drug auranofin from serum inactivation

This study shows that adding thiols to auranofin restores its anticancer activity by modulating albumin-gold binding and enhancing therapeutic efficacy.

Comparison and Evaluation of Neutralization of Clinically Frequently Used Antimicrobial Agents Using Three Different Culture Media in Simulated Blood Cultures

This study evaluates three blood culture media for antimicrobial neutralization, finding BACT/ALERT media outperformed others in recovery rates, while Thermo media had the lowest performance.

Role of Dusp5 KO on Vascular Properties of Middle Cerebral Artery in Rats

This study examines the structural and mechanical properties of the middle cerebral artery in Dusp5 KO rats, finding limited structural changes but enhanced myogenic tone, suggesting Dusp5's role in hemodynamics and cognition is enzyme-driven.

Dilated cardiomyopathy variant R14del increases phospholamban pentamer stability, blunting dynamic regulation of cardiac calcium handling

This study shows that the R14del mutation in phospholamban increases pentamer stability, reducing its ability to regulate SERCA and impairing cardiac calcium handling during physiological stress.

Decoding human in vitro terminal erythropoiesis originating from umbilical cord blood mononuclear cells and pluripotent stem cells

This study maps ex vivo terminal erythropoiesis from umbilical cord blood mononuclear cells and pluripotent stem cells, identifying differences in regulatory dynamics and CD99 expression affecting erythroid expansion and maturation.

An Unexpected Role of Neutrophils in Clearing Apoptotic Hepatocytes In Vivo

This study reveals that neutrophils, not macrophages, primarily clear apoptotic hepatocytes through a novel process called perforocytosis, and defects in this process contribute to autoimmune liver disease.

The soil emergence-related transcription factor PIF3 regulates root penetration by interacting with the receptor kinase FER

This study shows that PIF3, a transcription factor, regulates root penetration in Arabidopsis by interacting with FER, a receptor kinase, and modulating mechanosensitive ion channels and root cap cell sloughing.

NF-κB inhibitor alpha has a cross-variant role during SARS-CoV-2 infection in ACE2-overexpressing human airway organoids

The paper uses ACE2-overexpressing airway organoids to show NF-κB inhibitor alpha is upregulated during SARS-CoV-2 infection and its reduced function increases viral infection.

A genetically engineered phage-based nanomaterial for detecting bacteria with magnetic resonance imaging

This paper develops a genetically engineered phage-based nanomaterial for MRI detection of specific bacteria, demonstrating its ability to distinguish target strains from non-target bacteria and mammalian cells in vivo.

TME induced TRAIL secretion from engineered macrophages for anti-tumor therapy

This study engineers macrophages to inducibly secrete TRAIL in response to the tumor microenvironment, showing improved anti-tumor effects and reduced side effects in preclinical models.

Exosomally targeting microRNA23a ameliorates microvascular endothelial barrier dysfunction following rickettsial infection

This study shows that exosomal miR23a from rickettsial-infected endothelial cells disrupts BMEC barrier function by targeting ZO-1, and that anti-miR23a exosomes can restore barrier integrity.

Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants

This study shows that AtEH1 and AtEH2 subunits of the TPC complex drive biomolecular condensation to facilitate clathrin-mediated endocytosis in plants, with their disordered regions regulating condensate properties and endocytosis dynamics.

Oxidative phosphorylation pathway disruption is an alternative pathological mechanism leading to Diamond-Blackfan anemia

This study identifies oxidative phosphorylation pathway disruption as an alternative mechanism causing Diamond-Blackfan anemia and shows coenzyme Q10 can rescue erythroid defects.

Gut microbiota-derived tryptamine impairs insulin sensitivity

This study identifies tryptamine, a gut microbiota-derived metabolite, as a contributor to insulin resistance in type 2 diabetes through TAAR1-ERK signaling.

Structure of IgG-Fc hexamer reveals a mutual lock-and-key mode of Fc-Fc interaction

The paper determines the structure of an IgG-Fc hexamer using cryo-EM, revealing a mutual lock-and-key interaction mode between Fc domains.

Robust and Efficient Assessment of Potency (REAP): A Quantitative Tool for Dose-response Curve Estimation

The paper introduces a robust beta regression method for dose-response curve estimation and develops a web tool for its implementation.

Natural killer-like B cells promote Th17 cell differentiation and exacerbate rheumatoid arthritis

This study identifies natural killer-like B cells as pathogenic in rheumatoid arthritis by promoting Th17 differentiation and disease severity.

STOICHIOMETRY OF THE SODIUM PUMP-PHOSPHOLEMMAN REGULATORY COMPLEX

The paper investigates the stoichiometry and structure of the NKA-PLM regulatory complex using fluorescence lifetime assays and simulations, revealing a (alpha-beta-PLM)2 composition.

SMC1A facilitates gastric cancer cells proliferation, migration and invasion via promoting SNAI2 activated EMT

This study shows SMC1A promotes gastric cancer progression by enhancing SNAI2-driven EMT, suggesting it as a therapeutic target.

Construction of competing endogenous RNA interaction networks as prognostic markers in metastatic melanoma

This study constructs a ceRNA network in metastatic melanoma to identify prognostic markers and therapeutic targets, finding miR-3662 may be used in treatment.

Characterizing enterotypes in human metagenomics: a viral perspective

The paper analyzes human fecal virome data to identify viral enterotypes, finding two distinct patterns associated with health and disease.

ProXs drive the formation of membraneless organelles in plants

The paper identifies ProXs, proteins capable of forming membraneless organelles in plants, and shows their conservation across species.

The non-canonical interaction between calmodulin and calcineurin contributes to the differential regulation of plant-derived calmodulins on calcineurin.

This study investigates how plant-derived calmodulin variants differentially regulate calcineurin through non-canonical interactions beyond calcium binding and canonical CaMBR binding.

Inhibitory and Stimulatory Micropeptides Preferentially Bind to Different Conformations of the Cardiac Calcium Pump

The paper shows that inhibitory and stimulatory micropeptides bind to different conformations of the cardiac calcium pump SERCA, with their binding preference depending on intracellular Ca2+ levels.

HAUSP Stabilizes SOX2 through Deubiquitination to Maintain Self-renewal and Tumorigenic Potential of Glioma Stem Cells

This study identifies HAUSP as a deubiquitinase that stabilizes SOX2 in glioma stem cells, maintaining their self-renewal and tumorigenic potential, and shows that targeting HAUSP can enhance GBM treatment.

20-HETE-promoted cerebral blow flow autoregulation is associated with enhanced α-smooth muscle actin positive cerebrovascular pericyte contractility

This study shows that 20-HETE enhances pericyte contractility and cerebral blood flow autoregulation in rats by modulating vascular smooth muscle cell function.