W. Han

High-plex spatial RNA imaging in one round with conventional microscopes using color-intensity barcodes

This paper introduces PRISM, a method for high-plex spatial RNA imaging using color intensity barcodes, validated across multiple tissues and showing the role of CAFs in tumor microenvironments.

Defining cellular determinants of resistance to PD-1 pathway blockade in non-small-cell lung cancer

This study identifies MMP1+ fibroblasts as a cellular determinant of resistance to PD-1 pathway blockade in NSCLC by analyzing single-cell RNA sequencing data and spatial architecture.

Spatiotemporal control of subcellular O-GlcNAc signaling using Opto-OGT

This study introduces Opto-OGT, an optogenetic tool to control O-GlcNAc modification, showing its ability to modulate mTORC and AKT signaling in response to light.

Unraveling the gut microbiota of Tibetan chickens: insights into highland adaptation and ecological advantages

This study compares the gut microbiota of Tibetan chickens from different environments to identify adaptations to high-altitude conditions.

EPAS1 Attenuates Atherosclerosis Initiation at Disturbed Flow Sites through Endothelial Fatty Acid Uptake

This study shows EPAS1 reduces atherosclerosis at disturbed flow sites by promoting endothelial fatty acid uptake and metabolism, which is suppressed in obesity through triglyceride-PHD3 pathway.

GLP-1 Receptor Agonist Improves Metabolic Disease in a Pre-clinical Model of Lipodystrophy

This study shows that liraglutide, a GLP-1R agonist, improves metabolic disease in seipin knockout mice, a model of lipodystrophy, by enhancing insulin secretion and reducing liver fibrosis.

Epithelial Yap/Taz are required for functional alveolar regeneration following acute lung injury

This study shows that Yap/Taz in AT2 cells are essential for alveolar regeneration and preventing fibrosis after lung injury.

Repetitive Sulfur Dioxide Exposure in Mice Models Post-Deployment Respiratory Syndrome

This study develops a mouse model of repetitive sulfur dioxide exposure that mimics post-deployment respiratory syndrome features, identifying oxidative stress as a mediator of pulmonary vascular disease.

ScRNA-seq of Diverse Pheochromocytoma Patients Reveals Distinct Microenvironment Characteristics and Supports an Informative Molecular Classification System

This study uses scRNA-seq to classify PCCs into metabolism-type and kinase-type based on molecular features and microenvironment characteristics, suggesting potential therapeutic strategies.

Long-B prokaryotic Argonaute systems employ various effectors to confer immunity via abortive infection

The paper identifies long-B prokaryotic Argonaute systems as cell suicide mechanisms that use effector proteins to eliminate invading nucleic acids through abortive infection.

Pyruvate dehydrogenase kinase supports macrophage NLRP3 inflammasome activation during acute inflammation

This study shows that inhibiting PDHK reduces NLRP3 inflammasome activation in macrophages, decreasing inflammation and tissue damage.

miR-148a-3p inhibits osteogenesis by targeting Itga11 via PI3K/Akt/GSK3/β-catenin pathway

Extracellular matrix stiffness regulates fibroblast differentiation by influencing DNA methyltransferase 1 expression through microtubule polymerization

This study shows that extracellular matrix stiffness influences fibroblast differentiation by modulating DNMT1 expression via microtubule polymerization.

Molecular and genetic mechanisms conferring dissolution of dioecy in Diospyros oleifera Cheng

This study identifies molecular and genetic mechanisms underlying the transition from dioecy to monoecy in Diospyros oleifera using genome-wide analyses of 150 accessions.

Pathway and mechanism of tubulin folding mediated by TRiC/CCT conjugated with its ATPase cycle revealed by cryo-EM

This study reveals the pathway and mechanism of TRiC-mediated tubulin folding using cryo-EM structures and XL-MS, showing how tubulin is stabilized during the ATPase cycle.

Alveolar repair following lipopolysaccharide-induced injury requires cell-extracellular matrix interactions

This study shows that β1-containing integrins are essential for alveolar repair after injury, as their absence leads to emphysema and impaired differentiation of AT2 cells into mature type 1 alveolar epithelial cells.

Structural basis of plp2-mediated cytoskeletal protein folding by TRiC/CCT

This study uses cryo-EM to reveal structural details of TRiC/CCT-mediated folding of tubulin and actin with plp2, showing how plp2 coordinates substrate translocation and folding.

Predicting the antigenic evolution of SARS-COV-2 with deep learning

This paper predicts SARS-CoV-2 antigenic evolution using MLAEP, a deep learning model that identifies mutations associated with immune escape and validates them through experiments.

222-nm far UVC exposure results in DNA damage and transcriptional changes to mammalian cells.

This study compares the effects of 222-nm far UVC, 254-nm, and 277-nm UVC on mammalian cells, finding that 222-nm causes DNA damage and transcriptional changes despite cell survival.

Molecular basis of SARS-CoV-2 Omicron variant receptor engagement and antibody evasion and neutralization

This study investigates the structural basis of SARS-CoV-2 Omicron variant's immune evasion and transmissibility using cryo-EM, revealing conformational changes and enhanced ACE2 binding.

A short prokaryotic argonaute cooperates with membrane effector to confer antiviral defense

The paper identifies a short prokaryotic argonaute (pAgo) in Sulfolobus islandicus that, along with Aga1 and Aga2, provides antiviral defense through abortive infection.

Programmable RNA base editing with a single gRNA-free enzyme

This paper introduces a gRNA-free RNA editing system called REWIRE that achieves high editing efficiency and low off-target effects in human and mouse cells.

Enhanced soil quality after forest conversion to vegetable cropland and tea plantation has contrasting effects on soil microbial structure and functions

This study examines how soil quality changes after forest conversion to vegetable cropland and tea plantations, showing contrasting effects on microbial community structure and functions.

Variations in soil nutrient dynamics and bacterial communities in long-term tea monoculture production systems

This study examines how long-term tea monoculture affects soil nutrient dynamics and bacterial communities across different stand ages.

Cryo-EM study on the homo-oligomeric ring formation of yeast TRiC/CCT subunits reveals TRiC ring assembly mechanism

This study uses cryo-EM to reveal the mechanism of TRiC/CCT ring assembly in yeast, showing subunit-specific roles and ATP-driven assembly dynamics.

A novel mechanism of regulation of the transcription factor GLI3 by toll-like receptor signaling

This paper identifies TLR4-TRIF-IRF3 signaling as a novel pathway regulating GLI3 expression and its role in inflammatory cytokine secretion.

SUMO-specific protease 2 (SENP2) suppresses browning of white adipose tissue through C/EBPβ modulation

This study shows that SENP2 suppresses white adipose tissue browning by modulating C/EBPβ, leading to increased thermogenic gene expression and resistance to obesity in mice.

Imaging transmembrane dynamics of biomolecules at live cell plasma membranes using quenchers in extracellular environment

The paper develops a method to measure subnanometer position changes of biomolecules in live cell plasma membranes using extracellular quenchers and validates it by studying peptide-induced membrane perforation.

A Humanized Animal Model Predicts Clonal Evolution and Therapeutic Vulnerabilities in Myeloproliferative Neoplasms

This paper introduces a humanized animal model using patient-derived xenografts to study myeloproliferative neoplasms and identify therapeutic vulnerabilities.

The S1/S2 boundary of SARS-CoV-2 spike protein modulates cell entry pathways and transmission

This study shows that the S1/S2 boundary of the SARS-CoV-2 spike protein affects cell entry and transmission by altering the pathway used for viral entry.